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Impact of Silver and Iron Nanoparticle Exposure on Cholesterol Uptake by Macrophages.
Journal of Nanomaterials ( IF 3.791 ) Pub Date : 2015-10-18 , DOI: 10.1155/2015/127235
Jonathan H Shannahan 1 , Hari Sowrirajan 2 , Indushekhar Persaud 1 , Ramakrishna Podila 3, 4 , Jared M Brown 1
Affiliation  

Macrophages are central to the development of atherosclerosis by absorbing lipids, promoting inflammation, and increasing plaque deposition. Nanoparticles (NPs) are becoming increasingly common in biomedical applications thereby increasing exposure to the immune and vascular systems. This project investigated the influence of NPs on macrophage function and specifically cholesterol uptake. Macrophages were exposed to 20 nm silver NPs (AgNPs), 110 nm AgNPs, or 20 nm Fe3O4 NPs for 2 h and NP uptake, cytotoxicity, and subsequent uptake of fluorescently labeled cholesterol were assessed. Macrophage uptake of NPs did not induce cytotoxicity at concentrations utilized (25 μg/mL); however, macrophage exposure to 20 nm AgNPs reduced subsequent uptake of cholesterol. Further, we assessed the impact of a cholesterol-rich environment on macrophage function following NP exposure. In these sets of experiments, macrophages internalized NPs, exhibited no cytotoxicity, and altered cholesterol uptake. Alterations in the expression of scavenger receptor-B1 following NP exposure, which likely influences cholesterol uptake, were observed. Overall, NPs alter cholesterol uptake, which may have implications in the progression of vascular or immune mediated diseases. Therefore, for the safe development of NPs for biomedical applications, it is necessary to understand their impact on cellular function and biological interactions in underlying disease environments.

中文翻译:

银和铁纳米颗粒暴露对巨噬细胞摄取胆固醇的影响。

巨噬细胞通过吸收脂质,促进炎症和增加斑块沉积,对动脉粥样硬化的发展至关重要。纳米颗粒(NPs)在生物医学应用中变得越来越普遍,从而增加了对免疫和血管系统的暴露。该项目研究了NP对巨噬细胞功能特别是胆固醇摄取的影响。将巨噬细胞暴露于20 nm银NP(AgNP),110 nm AgNP或20 nm Fe 3 O 4 NP 2小时,并评估NP的摄取,细胞毒性和随后摄取的荧光标记胆固醇。在所使用的浓度下,巨噬细胞摄取NP不会引起细胞毒性(  25μg / mL); 然而,巨噬细胞暴露于20 nm AgNPs减少了随后胆固醇的摄取。此外,我们评估了暴露于NP后富含胆固醇的环境对巨噬细胞功能的影响。在这些实验组中,巨噬细胞将NP内在化,没有细胞毒性,并改变了胆固醇的摄取。观察到NP暴露后清道夫受体-B1的表达变化,这可能影响胆固醇的摄取。总体而言,NP会改变胆固醇的摄取,这可能对血管疾病或免疫介导的疾病的进展有影响。因此,为了安全地开发用于生物医学的NP,有必要了解其对基础疾病环境中细胞功能和生物相互作用的影响。
更新日期:2015-10-18
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