The hydrophobicity of 44 Vibrio strains isolated from cultured, diseased gilt-head sea bream (Sparus aurata) was determined. Three different methods were used: (1) microbial adhesion to hydrocarbons (MATH), either with phosphate buffer or with phosphate urea magnesium sulfate (PUM) buffer, (2) aggregation in the presence of salt solutions (SAT), and (3) adhesion to nitrocellulose filters (NCF). The results show that experimental conditions exerted a significant influence on hydrophobicity. Thus, Kendall rank coefficients showed the presence of correlation only for SAT and NCF, and for SAT and the MATH assay with PUM buffer. Moreover, no relationships were observed between the bacterial hydrophobicity estimated with the methods mentioned above and the ability of the strains to adhere to fish mucus or cells. These results indicate that adhesion of pathogenic Vibrio strains to host surfaces is mediated mainly by specific receptor interactions, instead of by hydrophobic interactions.

中文翻译：

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从受感染鱼中分离出的弧菌菌株对鱼细胞和粘液的疏水性和粘附性。

从养殖的，病态的金头鲷（Sparus aurata）分离出的44种弧菌菌株的疏水性 ）已确定。使用了三种不同的方法：（1）用磷酸盐缓冲液或磷酸盐尿素硫酸镁硫酸盐（PUM）缓冲液对微生物与碳氢化合物（MATH）的粘附；（2）在盐溶液（SAT）存在下聚集；以及（3）与硝酸纤维素滤膜（NCF）的粘附力。结果表明，实验条件对疏水性有重要影响。因此，Kendall秩系数显示仅对于SAT和NCF以及对于SAT和带有PUM缓冲液的MATH分析存在相关性。此外，在用上述方法估计的细菌疏水性与菌株粘附于鱼粘液或细胞的能力之间未观察到任何关系。这些结果表明，致病性弧菌的粘附 寄主表面的毒株主要通过特异性受体相互作用而不是通过疏水相互作用介导。