The attachment of implants relies on bone and soft tissue biocompatibility. The aim of this article is to investigate the effect of fluoride-modified metallic titanium (Ti) surfaces (Ti-F) on proliferation and differentiation of human gingival fibroblasts. Human gingival fibroblast cells were exposed to hydrofluoric acid-modified Ti coins (Ti-F) for 1, 3, 7, 14 and 21 days, and untreated coins were used as controls. A five- to six-fold increase in the proliferation of human gingival fibroblasts on Ti-F compared to Ti surfaces was observed. Enhanced gene expression of interleukin-6 and osteoprotegerin was found at 7 days. Increased levels of sclerostin, interleukin-6 and osteoprotegerin in the media from human gingival fibroblasts cultured on Ti-F coins were found compared to controls. Our results confirm that hydrofluoric acid-modified surface may indirectly enhance the firm attachment of implant surface to junction epithelium, soft tissue epithelium, which would give protection for underlying osseous structures making osseointegration of the dental implant possible.

中文翻译：

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钛表面的氢氟酸处理可增强人类牙龈成纤维细胞的增殖。

植入物的附着取决于骨骼和软组织的生物相容性。本文的目的是研究氟化物修饰的金属钛（Ti）表面（Ti-F）对人牙龈成纤维细胞增殖和分化的影响。将人牙龈成纤维细胞暴露于氢氟酸修饰的Ti硬币（Ti-F）中1、3、7、14和21天，未处理的硬币用作对照。与钛表面相比，观察到人类牙龈成纤维细胞在Ti-F上的增殖增加了5至6倍。在第7天发现白介素6和骨保护素的基因表达增强。与对照相比，发现在Ti-F硬币上培养的人牙龈成纤维细胞培养基中的硬化蛋白，白介素6和骨保护素水平增加。