This study demonstrates that cell wall treatment of Lactococcus lactis harbouring the internal ribosome entry site-incorporated lactococcal bicistronic vector pNZ:VIG mediated the delivery of genes into an eukaryotic cell line, DF1 cells, through bactofection. Bactofection analysis showed that the pNZ:VIG plasmid in L. lactis can be transferred into DF1 cells and that both the VP2 and gfp genes cloned in the plasmid can be transcribed and translated. The protein band relative to the Mr of VP2 protein (49 kDa) was successfully detected via Western blot analysis, while green fluorescence was successfully detected using a fluorescence microscope. The intensity of the bands detected increased for samples treated with both 1.5% (w/v) glycine and 10 μg/mL of lysozyme when compared to L. lactis treated with glycine alone and without treatment. Cell wall treatment of L. lactis with a combination of both glycine and lysozyme was not only shown to mediate plasmid transfer to DF1 cells, but also to increase the plasmid transfer efficiency.

中文翻译：

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细胞壁处理的乳酸乳球菌可增加内部核糖体进入位点的乳球菌双顺反子载体进入DF1细胞的质粒转移效率。

这项研究表明，带有内核糖体进入位点并入乳球菌双顺反子载体pNZ：VIG的乳酸乳球菌的细胞壁处理可通过细菌感染介导基因传递到真核细胞系DF1细胞中。细菌感染分析表明，乳酸乳球菌中的pNZ：VIG质粒可以转移到DF1细胞中，克隆在质粒中的VP2和gfp基因都可以被转录和翻译。通过蛋白质印迹分析成功检测到相对于VP2蛋白Mr的蛋白带（49 kDa），而使用荧光显微镜成功检测到绿色荧光。与仅用甘氨酸处理而未经处理的乳酸乳球菌相比，用1.5％（w / v）甘氨酸和10μg/ mL溶菌酶处理的样品检测到的条带强度增加。