Anin vitro selection system using microcross sections of banana and plantain cultivars belonging to AAA and AAB genomic groups were used to produce plants resistant against the Black Sigatoka disease. The fungus resistant plantlets were obtained in a double selection system. This involved in a first step the use of a fungal crude filtrate and in the second step the purified host-specific toxin 2,4,8-trihydroxytetralone extracted from the fungusMycosphaerella fijiensis (M. fijiensis), the causal agent of Black Sigatoka disease. Resistant plantlets obtained from the double selection system were inoculated with conidia ofM. fijiensis in a growth chamber to reproduce Black Sigatoka symptoms. Compared to non-treated control plantlets, which were highly susceptible to the fungus, 10.7-19.3% toxin-resistant plantlets which arose from tissues that went through the double selection system were resistant againstM. fijiensis. This technique of using micro-cross sections for selection on fungal toxins seems to be amenable to differentMusa genotypes for the production of fungus-resistant plants.

中文翻译：

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从香蕉和车前草的微截面中选择斐济支原体的细胞系。

使用香蕉和车前草属AAA和AAB基因组的微型横切面的体外选择系统来产生抗Black Sigatoka病的植物。在双重选择系统中获得了抗真菌苗。这第一步涉及使用真菌粗滤液，第二步涉及从黑S病的病原体真菌Mycosphaerella fijiensis（M. fijiensis）提取的纯化的宿主特异性毒素2,4,8-三羟基四氢萘酮。用M分生孢子接种从双重选择系统获得的抗性小苗。fijiensis在生长室中繁殖黑色Sigatoka症状。与高度处理真菌的未处理对照小苗相比，为10.7-19。来自经过双重选择系统的组织产生的3％的抗毒素小苗对M具有抗性。斐济 使用微截面选择真菌毒素的这项技术似乎适合于不同的Musa基因型来生产抗真菌植物。