During development, axonogenesis, an integral part of neurogenesis, is based on well-concerted events comprising generation, rearrangement, migration, elongation, and adhesion of neurons. Actin, specifically the crosstalk between the guardians of actin polymerization, like enabled, chickadee, capping protein plays an essential role in crafting several events of axonogenesis. Recent evidences reflect multifaceted role of microRNA during axonogenesis. Here, we investigated the role of bantam miRNA, a well-established miRNA in Drosophila, in regulating the actin organization during brain development. Our immunofluorescence studies showed altered arrangement of neurons and actin filaments whereas both qPCR and western blot revealed elevated expression of enabled, one of the actin modulators in bantam mutant background. Collectively, our results clearly demonstrate that bantam plays an instrumental role in shaping the axon architecture regulating the actin geometry through its modulator enabled.

中文翻译：

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矮脚鸡通过调节肌动蛋白调节剂来调节果蝇幼虫大脑的轴突几何形状。

在发育过程中，轴突发生是神经发生的组成部分，它基于公认的事件，包括神经元的生成，重排，迁移，伸长和粘附。肌动蛋白，特别是肌动蛋白聚合的守护者之间的串扰，如使能的，山雀，封端蛋白，在拟定轴突发生的若干事件中起着至关重要的作用。最近的证据反映了microRNA在轴突发生过程中的多方面作用。在这里，我们调查了果蝇中成熟的miRNA矮脚鸡miRNA在调节大脑发育过程中肌动蛋白组织中的作用。我们的免疫荧光研究表明，神经元和肌动蛋白丝的排列方式发生了变化，而qPCR和Western blot均显示了矮脚鸡肌动蛋白调节剂之一的使能蛋白的表达升高。突变体背景。总的来说，我们的结果清楚地表明，矮脚鸡在塑造轴突结构中发挥了重要作用，该轴突结构通过启用的调节剂来调节肌动蛋白的几何形状。