BACKGROUND In this work we have determined molecular signatures of oviduct epithelial and progenitor cells. We have proposed a panel of selected marker genes, which correspond with the phenotype of oviduct cells of a laying hen (Gallus gallus domesticus) and quail (Coturnix japonica). We demonstrated differences in characteristics of those cells, in tissue and in vitro, with respect to different anatomical and functional parts of the oviduct (infundibulum (INF), distal magnum (DM, and proximal magnum (PM)). The following gene expression signatures were studied: (1) oviduct markers (estrogen receptor 1, ovalbumin, and SPINK7 - ovomucoid), (2) epithelial markers (keratin 5, keratin 14, and occludin) and (3) stem-like/progenitor markers (CD44 glycoprotein, LGR5, Musashi-1, and sex determining region Y-box 9, Nanog homebox, OCT4/cPOUV gene encoding transcription factor POU5F3). RESULTS In chicken, the expression of oviduct markers increased toward the proximal oviduct. Epithelial markers keratin14 and occludin were high in distal oviduct and decreased toward the proximal magnum. In quail oviduct tissue, the gene expression pattern of oviduct/epithelial markers was similar to chicken. The markers of progenitors/stemness in hen oviduct (Musashi-1 and CD44 glycoprotein) had the highest relative expression in the infundibulum and decreased toward the proximal magnum. In quail, we found significant expression of four progenitor markers (LGR5 gene, SRY sex determining region Y-box 9, OCT4/cPOUV gene, and CD44 glycoprotein) that were largely present in the distal oviduct. After in vitro culture of oviduct cells, the gene expression pattern has changed. High secretive potential of magnum-derived cells diminished by using decreased abundance of mRNA. On the other hand, chicken oviduct cells originating from the infundibulum gained ability to express OVM and OVAL. Epithelial character of the cells was maintained in vitro. Among progenitor markers, both hen and quail cells expressed high level of SOX9, LGR5 and Musashi-1. CONCLUSION Analysis of tissue material revealed gradual increase/decrease pattern in majority of the oviduct markers in both species. This pattern changed after the oviductal cells have been cultured in vitro. The results can provide molecular tools to validate the phenotype of in vitro biological models from reproductive tissue.

中文翻译：

---

蛋鸡（Gallus gallus domesticus）和鹌鹑（Coturnix japonica）的上皮输卵管细胞的分子标记。

背景技术在这项工作中，我们确定了输卵管上皮细胞和祖细胞的分子标记。我们提出了一组选定的标记基因，这些基因与蛋鸡（Gallus gallus domesticus）和鹌鹑（Coturnix japonica）的输卵管细胞表型相对应。我们证明了这些细胞在组织和体外的特性在输卵管的不同解剖和功能部位（漏斗（INF），远端大瓶（DM）和近端大瓶（PM））方面存在差异。进行了以下研究：（1）输卵管标记物（雌激素受体1，卵清蛋白和SPINK7-卵粘液），（2）上皮标记物（角蛋白5，角蛋白14和锁骨蛋白）和（3）茎样/祖细胞标记物（CD44糖蛋白， LGR5，Musashi-1和性别确定区域Y-box 9，Nanog homebox，OCT4 / cPOUV基因编码转录因子POU5F3）。结果在鸡中，输卵管标志物的表达向近端输卵管增加。上皮标志物keratin14和occludin在远端输卵管中较高，并向近端大瓶中减少。在鹌鹑输卵管组织中，输卵管/上皮标记的基因表达模式与鸡相似。输卵管中的祖细胞/干细胞标志物（Musashi-1和CD44糖蛋白）在漏斗骨中具有最高的相对表达，并向近端大指骨减少。在鹌鹑中，我们发现在远端输卵管中大量存在的四个祖细胞标记物（LGR5基因，SRY性别决定区Y-box 9，OCT4 / cPOUV基因和CD44糖蛋白）表达显着。输卵管细胞体外培养后，基因表达模式发生了变化。使用减少的mRNA丰度可降低大细胞来源细胞的高分泌潜能。另一方面，源自漏斗的鸡输卵管细胞获得了表达OVM和OVAL的能力。在体外维持细胞的上皮特性。在祖细胞标记中，母鸡和鹌鹑细胞均表达高水平的SOX9，LGR5和Musashi-1。结论对组织材料的分析表明，两种物种中大多数输卵管标记物的逐渐增加/减少方式。在输卵管细胞进行体外培养后，这种模式发生了变化。结果可以提供分子工具，以验证生殖组织的体外生物学模型的表型。来自漏斗的鸡输卵管细胞获得了表达OVM和OVAL的能力。在体外维持细胞的上皮特性。在祖细胞标记中，母鸡和鹌鹑细胞均表达高水平的SOX9，LGR5和Musashi-1。结论对组织材料的分析表明，两种物种中大多数输卵管标记物的逐渐增加/减少方式。在输卵管细胞进行体外培养后，这种模式发生了变化。结果可以提供分子工具，以验证生殖组织的体外生物学模型的表型。来自漏斗的鸡输卵管细胞获得了表达OVM和OVAL的能力。在体外维持细胞的上皮特性。在祖细胞标记中，母鸡和鹌鹑细胞均表达高水平的SOX9，LGR5和Musashi-1。结论对组织材料的分析表明，两种物种中大多数输卵管标记物的逐渐增加/减少方式。在输卵管细胞进行体外培养后，这种模式发生了变化。结果可以提供分子工具，以验证生殖组织的体外生物学模型的表型。结论对组织材料的分析表明，两种物种中大多数输卵管标记物的逐渐增加/减少方式。在输卵管细胞进行体外培养后，这种模式发生了变化。结果可以提供分子工具，以验证生殖组织的体外生物学模型的表型。结论对组织材料的分析表明，两种物种中大多数输卵管标记物的逐渐增加/减少方式。在输卵管细胞进行体外培养后，这种模式发生了变化。结果可以提供分子工具，以验证生殖组织的体外生物学模型的表型。