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The Current Status and Future Outlook of Quantum Dot-Based Biosensors for Plant Virus Detection.
The Plant Pathology Journal ( IF 2.3 ) Pub Date : 2018-04-01 , DOI: 10.5423/ppj.rw.08.2017.0184
Sungyeap Hong 1 , Cheolho Lee 1
Affiliation  

Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), widely used for the detection of plant viruses, are not easily performed, resulting in a demand for an innovative and more efficient diagnostic method. This paper summarizes the characteristics and research trends of biosensors focusing on the physicochemical properties of both interface elements and bioconjugates. In particular, the topological and photophysical properties of quantum dots (QDs) are discussed, along with QD-based biosensors and their practical applications. The QD-based Fluorescence Resonance Energy Transfer (FRET) genosensor, most widely used in the biomolecule detection fields, and QD-based nanosensor for Rev-RRE interaction assay are presented as examples. In recent years, QD-based biosensors have emerged as a new class of sensor and are expected to open opportunities in plant virus detection, but as yet there have been very few practical applications (Table 3). In this article, the details of those cases and their significance for the future of plant virus detection will be discussed.

中文翻译:

基于量子点的植物病毒检测生物传感器的现状与展望。

不易进行广泛用于检测植物病毒的酶联免疫吸附测定(ELISA)和聚合酶链反应(PCR),因此需要一种创新,更有效的诊断方法。本文总结了生物传感器的特点和研究趋势,重点研究了界面元素和生物结合物的理化性质。特别是,讨论了量子点(QD)的拓扑和光物理特性,以及基于QD的生物传感器及其实际应用。以基于QD的荧光共振能量转移(FRET)基因传感器(在生物分子检测领域中使用最广泛)以及基于QD的纳米传感器用于Rev-RRE相互作用测定为例。最近几年,基于量子点的生物传感器已经成为一类新型的传感器,并有望为植物病毒检测打开机会,但到目前为止,实际应用还很少(表3)。在本文中,将讨论这些案例的详细信息及其对未来植物病毒检测的意义。
更新日期:2020-08-21
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