Imaging Fourier-transform spectroscopy (IFTS) is a powerful method for biological hyperspectral analysis based on various imaging modalities, such as fluorescence or Raman. Since the measurements are taken in the Fourier space of the spectrum, it can also take advantage of compressed sensing strategies. IFTS has been readily implemented in high-throughput, high-content microscope systems based on wide-field imaging modalities. However, there are limitations in existing wide-field IFTS designs. Non-common-path approaches are less phase-stable. Alternatively, designs based on the common-path Sagnac interferometer are stable, but incompatible with high-throughput imaging. They require exhaustive sequential scanning over large interferometric path delays, making compressive strategic data acquisition impossible. In this paper, we present a novel phase-stable, near-common-path interferometer enabling high-throughput hyperspectral imaging based on strategic data acquisition. Our results suggest that this approach can improve throughput over those of many other wide-field spectral techniques by more than an order of magnitude without compromising phase stability.

中文翻译：

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宽视场显微镜中用于傅立叶变换光谱成像的近共径干涉仪。

成像傅里叶变换光谱（IFTS）是一种基于各种成像模式（例如荧光或拉曼光谱）的生物高光谱分析的强大方法。由于测量是在频谱的傅立叶空间中进行的，因此它也可以利用压缩传感策略。IFTS已很容易在基于宽视场成像模式的高通量，高含量显微镜系统中实现。但是，现有的广域IFTS设计存在局限性。非公共路径方法的相位稳定性较差。另外，基于公共路径Sagnac干涉仪的设计是稳定的，但与高通量成像不兼容。它们需要在较大的干涉路径延迟上进行详尽的顺序扫描，从而无法进行压缩的战略数据采集。在本文中，我们提出了一种新型的相位稳定，近共径干涉仪，该干涉仪能够基于战略数据采集实现高通量高光谱成像。我们的结果表明，与其他许多广域光谱技术相比，该方法可以将吞吐量提高一个数量级以上，而不会损害相位稳定性。