We previously reported that the terminal differentiation of odontoblasts was inhibited in Runx2 transgenic {Tg(Col1a1-Runx2)} mice under the control of the 2.3-kb Col1a1 promoter. Odontoblasts in Tg(Col1a1-Runx2) mice lose their characteristic long cellular processes, and show marked reductions in the protein levels of markers for odontoblasts, such as dentin sialophosphoprotein, nestin, and microtubule-associated protein tau (Mapt). We herein demonstrated that collapsin response mediator protein 1 (CRMP1), a neuronal phosphoprotein that participates in various aspects of neuronal development, was specifically expressed in the differentiated odontoblasts of wild-type, but not Tg(Col1a1-Runx2) tooth germs by comparing expression profiles in wild-type and Tg(Col1a1-Runx2) mouse molars using microarray and immunohistochemical analyses. CRMP1 expression was detected at a slightly later differentiation stage in odontoblasts than type 1 collagen, nestin, and Mapt expression, which was observed from the onset of dentinogenesis. Among these proteins, CRMP1 was the most specifically localized in odontoblasts in the tooth germ. In erupted molars, odontoblast-specific CRMP1 expression decreased with age. These results indicate that CRMP1 is a novel marker protein for differentiated odontoblasts in mouse tooth germs, and suggest that CRMP1 participates in the morphogenesis of functioning odontoblasts.

中文翻译：

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胶原蛋白介导蛋白1，一种用于分化成牙本质细胞的新型标记蛋白。

我们以前曾报道在2.3-kb Col1a1启动子的控制下，Runx2转基因{Tg（Col1a1-Runx2）}小鼠中成牙本质细胞的终末分化受到抑制。Tg（Col1a1-Runx2）小鼠的成牙本质细胞失去其特征性的长细胞过程，并显示成牙本质细胞标记物（例如牙本质唾液磷蛋白，巢蛋白和微管相关蛋白tau，Mapt）的蛋白质水平显着降低。我们在这里证明，通过比较表达，collapsin反应介质蛋白1（CRMP1），一种参与神经元发育各个方面的神经元磷酸蛋白，在分化的野生型成牙本质细胞中表达，但在Tg（Col1a1-Runx2）牙胚中没有表达使用微阵列和免疫组织化学分析野生型和Tg（Col1a1-Runx2）小鼠磨牙中的蛋白谱。在成牙本质细胞的分化阶段中检测到CRMP1的表达要比1型胶原蛋白，巢蛋白和Mapt的表达稍晚一些，这是从牙本质发生开始就观察到的。在这些蛋白质中，CRMP1最明确地位于牙齿胚芽的成牙本质细胞中。在磨牙中，成牙本质细胞特定的CRMP1表达随年龄下降。这些结果表明CRMP1是小鼠牙胚中分化的成牙本质细胞的新型标记蛋白，并表明CRMP1参与了功能正常的成牙本质细胞的形态发生。在磨牙中，成牙本质细胞特定的CRMP1表达随年龄下降。这些结果表明CRMP1是小鼠牙胚中分化的成牙本质细胞的新型标记蛋白，并表明CRMP1参与了功能正常的成牙本质细胞的形态发生。在磨牙中，成牙本质细胞特定的CRMP1表达随年龄下降。这些结果表明CRMP1是小鼠牙胚中分化的成牙本质细胞的新型标记蛋白，并表明CRMP1参与了功能正常的成牙本质细胞的形态发生。