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Progression of phosphine resistance in susceptible Tribolium castaneum (Herbst) populations under different immigration regimes and selection pressures.
Evolutionary Applications ( IF 4.1 ) Pub Date : 2017-06-14 , DOI: 10.1111/eva.12493
Michelle A Rafter 1 , Graham A McCulloch 1 , Gregory J Daglish 2 , Gimme H Walter 1
Affiliation  

Insecticide resistance is an escalating global issue for a wide variety of agriculturally important pests. The genetic basis and biochemical mechanisms of resistance are well characterized in some systems, but little is known about the ecological aspects of insecticide resistance. We therefore designed a laboratory experiment to quantify the progression of phosphine resistance in Tribolium castaneum populations subject to different immigration regimes and selection pressures. Mated resistant females were added to originally susceptible populations under two distinct migration rates, and in addition, half of the populations in each migration treatment were exposed to selection pressures from phosphine fumigation. The progression of phosphine resistance was assessed by screening beetles for the resistance allele at rph2. Phosphine resistance increased slowly in the low migration treatment and in the absence of selection, as expected. But at the higher migration rate, the increase in frequency of the resistance allele was lower than predicted. These outcomes result from the high levels of polyandry known in T. castaneum females in the laboratory, because most of the Generation 1 offspring (86%) were heterozygous for the rph2 allele, probably because resistant immigrant females mated again on arrival. Phosphine resistance was not fixed by fumigation as predicted, perhaps because susceptible gametes and eggs survived fumigation within resistant females. In terms of phosphine resistance progression in populations exposed to selection, the effect of fumigation negated the difference in migration rates. These results demonstrate how species‐specific traits relating to the mating system may shape the progression of insecticide resistance within populations, and they have broad implications for the management of phosphine resistance in T. castaneum in the field. ​We specify and discuss how these mating system attributes need to be accounted for when developing guidelines for resistance management.

中文翻译:

在不同的移民制度和选择压力下易感的bol藜(Herbst)种群对膦的抗性进展。

杀虫剂的耐药性是各种对农业重要的害虫日益严重的全球性问题。抗药性的遗传基础和生化机理在某些系统中已得到很好的表征,但对杀虫剂抗性的生态学方面知之甚少。因此,我们设计了一个实验室实验,以定量测定蓖麻毒Tri中膦抗性的进程受不同移民制度和选择压力的人口。以两种不同的迁移率将抗性交配的雌性添加到最初易感的种群中,此外,每种迁移处理中的一半种群都遭受了磷化氢熏蒸的选择压力。通过筛选甲虫在rph 2处的抗性等位基因来评估膦抗性的进展。如所预期的,在低迁移处理和无选择的情况下,膦抗性缓慢增加。但是在较高的迁移率下,抗性等位基因频率的增加低于预期。这些成果从高层次一妻多夫已知导致赤拟谷盗实验室中的雌性,因为大多数第一代后代(86%)对rph2 等位基因是杂合的 ,可能是因为抗性移民雌性在到达时再次交配。膦的抗性并不能像预期的那样通过熏蒸来固定,这可能是因为易感的配子和卵在抗性雌性的熏蒸中得以幸存。就暴露于选择的人群的膦抗性进展而言,熏蒸的效果抵消了迁移率的差异。这些结果证明了与交配系统有关的特定物种性状如何影响种群内杀虫剂抗性的发展,并且它们对锥栗草的耐膦性具有广泛的影响 在该领域。``我们指定并讨论在制定抗性管理指南时如何考虑这些交配系统属性。
更新日期:2017-06-14
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