Hydrolytic enzymes are a large class of biological catalysts that play a vital role in a plethora of critical biochemical processes required to maintain human health. However, the expression and/or activity of these important enzymes can change in many different diseases and therefore represent exciting targets for the development of positron emission tomography (PET) and single-photon emission computed tomography (SPECT) radiotracers. This review focuses on recently reported radiolabeled substrates, reversible inhibitors, and irreversible inhibitors investigated as PET and SPECT tracers for imaging hydrolytic enzymes. By learning from the most successful examples of tracer development for hydrolytic enzymes, it appears that an early focus on careful enzyme kinetics and cell-based studies are key factors for identifying potentially useful new molecular imaging agents.

中文翻译：

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使用PET和SPECT对水解酶进行分子成像。

水解酶是一大类生物催化剂，在维持人类健康所需的大量关键生化过程中起着至关重要的作用。但是，这些重要酶的表达和/或活性可能在许多不同的疾病中发生变化，因此代表了正电子发射断层扫描（PET）和单光子发射计算机断层扫描（SPECT）放射性示踪剂发展的令人兴奋的目标。这篇综述着重于最近报道的放射性标记底物，可逆抑制剂和不可逆抑制剂，它们被用作成像水解酶的PET和SPECT示踪剂。通过学习最成功的水解酶示踪剂开发实例，