Multiphoton microscopy has emerged as a ubiquitous tool for studying microscopic structure and function across a broad range of disciplines. As such, the intent of this paper is to present a comprehensive resource for the construction and performance evaluation of a multiphoton microscope that will be understandable to the broad range of scientific fields that presently exploit, or wish to begin exploiting, this powerful technology. With this in mind, we have developed a guide to aid in the design of a multiphoton microscope. We discuss source selection, optical management of dispersion, image-relay systems with scan optics, objective-lens selection, single-element light-collection theory, photon-counting detection, image rendering, and finally, an illustrated guide for building an example microscope.

中文翻译：

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多光子显微镜设计实用指南

多光子显微镜已成为跨学科研究微观结构和功能的普遍工具。因此，本文的目的是为多光子显微镜的构建和性能评估提供全面的资源，目前正在利用或希望开始利用这种强大技术的广泛科学领域都可以理解该资源。考虑到这一点，我们开发了一份指南来帮助设计多光子显微镜。我们讨论光源选择、色散光学管理、具有扫描光学器件的图像中继系统、物镜选择、单元件光收集理论、光子计数检测、图像渲染，最后，构建示例显微镜的图解指南。