Annigeri 1 and JG 74 are elite high yielding desi cultivars of chickpea with medium maturity duration and extensively cultivated in Karnataka and Madhya Pradesh, respectively. Both cultivars, in recent years, have become susceptible to race 4 of Fusarium wilt (FW). To improve Annigeri 1 and JG 74, we introgressed a genomic region conferring resistance against FW race 4 (foc4) through marker-assisted backcrossing using WR 315 as the donor parent. For foreground selection, TA59, TA96, TR19 and TA27 markers were used at Agricultural Research Station, Kalaburagi, while GA16 and TA96 markers were used at Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur. Background selection using simple sequence repreats (SSRs) for the cross Annigeri 1 × WR 315 in BC1F1 and BC2F1 lines resulted in 76-87% and 90-95% recurrent parent genome recovery, respectively. On the other hand, 90-97% genome was recovered in BC3F1 lines in the case of cross JG 74 × WR 315. Multilocation evaluation of 10 BC2F5 lines derived from Annigeri 1 provided one superior line referred to as Super Annigeri 1 with 8% increase in yield and enhanced disease resistance over Annigeri 1. JG 74315-14, the superior line in JG 74 background, had a yield advantage of 53.5% and 25.6% over the location trial means in Pantnagar and Durgapura locations, respectively, under Initial Varietal Trial of All India Coordinated Research Project on Chickpea. These lines with enhanced resistance and high yield performance are demonstration of successful deployment of molecular breeding to develop superior lines for FW resistance in chickpea.

中文翻译：

---

Super Annigeri 1 和改进的 JG 74：使用标记辅助回交方法在鹰嘴豆 (Cicer arietinum L.) 中开发的两个抗镰刀菌枯萎病渗入系。

Annigeri 1 和 JG 74 是鹰嘴豆的优良高产德西栽培品种，具有中等成熟期，分别在卡纳塔克邦和中央邦广泛种植。近年来，这两个品种都对枯萎病 (FW) 小种 4 敏感。为了改进 Annigeri 1 和 JG 74，我们使用 WR 315 作为供体亲本，通过标记辅助回交引入了一个基因组区域，该区域赋予对 FW 种族 4 (foc4) 的抗性。对于前景选择，Kalaburagi 农业研究站使用了 TA59、TA96、TR19 和 TA27 标记，而贾巴尔普尔 Jawaharlal Nehru Krishi Vishwa Vidyalaya 使用了 GA16 和 TA96 标记。在 BC1F1 和 BC2F1 系中使用简单序列重复 (SSR) 进行交叉 Annigeri 1 × WR 315 的背景选择分别导致 76-87% 和 90-95% 的轮回亲本基因组恢复。另一方面，在杂交 JG 74 × WR 315 的情况下，BC3F1 系中恢复了 90-97% 的基因组。对源自 Annigeri 1 的 10 个 BC2F5 系进行多位置评估，提供了一个被称为超级 Annigeri 1 的优良系，增加了 8%与 Annigeri 1 相比，产量和抗病性增强。在初始品种试验中，JG 74315-14（JG 74 背景中的优良品系）的产量优势分别比 Pantnagar 和 Durgapura 地点的地点试验平均值高 53.5% 和 25.6%全印度鹰嘴豆协调研究项目。这些具有增强的抗性和高产性能的品系证明了分子育种的成功部署，以开发鹰嘴豆 FW 抗性的优良品系。