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Enhanced Osteoblast Response to Porosity and Resolution of Additively Manufactured Ti-6Al-4V Constructs with Trabeculae-Inspired Porosity.
3D Printing and Additive Manufacturing ( IF 3.1 ) Pub Date : 2016-03-01 , DOI: 10.1089/3dp.2015.0038
Alice Cheng 1, 2, 3 , Aiza Humayun 4 , Barbara D Boyan 1, 4 , Zvi Schwartz 4, 5
Affiliation  

The addition of porosity to the traditionally used solid titanium metal implants has been suggested to more closely mimic the natural mechanical properties of bone and increase osseointegration in dental and orthopedic implants. The objective of this study was to evaluate cellular response to three-dimensional (3D) porous Ti-6Al-4V constructs fabricated by additive manufacturing using laser sintering with low porosity (LP), medium porosity (MP), and high porosity (HP) with low resolution (LR) and high resolution (HR) based on a computed tomography scan of human trabecular bone. After surface processing, construct porosity ranged from 41.0% to 76.1%, but all possessed micro-/nanoscale surface roughness and similar surface chemistry containing mostly Ti, O, and C. Biological responses (osteoblast differentiation, maturation, and local factor production) by MG63 osteoblast-like cells and normal human osteoblasts favored 3D than two-dimensional (2D) solid constructs. First, MG63 cells were used to assess differences in cell response to 2D compared to LR and HR porous 3D constructs. MG63 cells were sensitive to porosity resolution and exhibited increased osteocalcin (OCN), vascular endothelial growth factor (VEGF), osteoprotegerin (OPG), and bone morphogenetic protein 2 (BMP2) on HR 3D constructs than on 2D and LR 3D constructs. MG63 cells also exhibited porosity-dependent responses on HR constructs, with up to a 6.9-fold increase in factor production on LP-HR and MP-HR constructs than on HP-HR constructs. NHOsts were then used to validate biological response on HR constructs. NHOsts exhibited decreased DNA content and alkaline phosphatase activity and up to a 2.9-fold increase in OCN, OPG, VEGF, BMP2, and BMP4 on 3D HR constructs than on 2D controls. These results indicate that osteoblasts prefer a 3D architecture than a 2D surface and that osteoblasts are sensitive to the resolution of trabecular detail and porosity parameters of laser-sintered 3D Ti-6Al-4V constructs.

中文翻译:

增强的成骨细胞对孔隙度的响应,以及由小梁启发的孔隙度的增材制造的Ti-6Al-4V构造的拆分。

已建议在传统使用的固态钛金属植入物中增加孔隙率,以更紧密地模仿骨骼的自然机械性能,并增加牙科和整形外科植入物中的骨整合。这项研究的目的是评估对采用低孔隙度(LP),中孔隙度(MP)和高孔隙率(HP)的激光烧结通过增材制造制造的三维(3D)多孔Ti-6Al-4V结构的细胞响应基于人眼小梁的计算机断层扫描技术,具有低分辨率(LR)和高分辨率(HR)。经过表面处理后,构造物的孔隙率在41.0%至76.1%的范围内,但都具有微米/纳米级的表面粗糙度和相似的表面化学成分,主要包含Ti,O和C。生物学响应(成骨细胞分化,成熟,MG63成骨细胞样细胞和正常人成骨细胞比3D二维(2D)固体构建体更青睐3D。首先,与LR和HR多孔3D构建体相比,MG63细胞用于评估细胞对2D响应的差异。MG63细胞对孔隙度分辨率敏感,并且与2D和LR 3D构建体相比,HR 3D构建体的骨钙素(OCN),血管内皮生长因子(VEGF),骨保护素(OPG)和骨形态发生蛋白2(BMP2)有所增加。MG63细胞还对HR构造物表现出孔隙率依赖性反应,与HP-HR构造物相比,LP-HR和MP-HR构造物产生的因子最多增加6.9倍。然后将NHOsts用于验证对HR构建体的生物学反应。NHOsts的DNA含量和碱性磷酸酶活性降低,最高可达2。3D HR构建体的OCN,OPG,VEGF,BMP2和BMP4的增加是2D对照的9倍。这些结果表明,成骨细胞比2D表面更喜欢3D结构,并且成骨细胞对激光烧结的3D Ti-6Al-4V结构的小梁细节分辨率和孔隙率参数敏感。
更新日期:2019-11-01
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