Cisplatin is used to treat a variety of tumors, but dose-limiting toxicities or intrinsic and acquired resistance limit its application in many types of cancer including breast. Cisplatin was attached to silica nanoparticles using aminopropyltriethoxy silane as a linker molecule and characterized in terms of size, shape, as well as the dissolution of cisplatin from the silica surface. The primary particle diameter of the as received silica nanoparticles ranged from 20 to 90 nm. The results show that adverse effects on cell function, as evidenced by reduced metabolic activity measured by the MTT assay and increased membrane permeability observed using the live/dead stain, can be correlated with surface area of the silica. Cisplatin-functionalized silica nanoparticles with the highest surface area incited the greatest response, which was almost equivalent to that induced by free cisplatin. Moreover, if verified by further studies, would indicate that cisplatin was attached to silica nanoparticles might prove to be useful in site-specific drug delivery.

中文翻译：

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用于癌症化疗的顺铂官能化二氧化硅纳米粒子。

顺铂用于治疗多种肿瘤，但剂量限制性毒性或内在性和获得性耐药性限制了它在许多类型的癌症（包括乳腺癌）中的应用。使用氨丙基三乙氧基硅烷作为连接分子将顺铂连接到二氧化硅纳米颗粒上，并根据大小，形状以及顺铂从二氧化硅表面的溶解进行表征。所接收的二氧化硅纳米颗粒的初级粒径为20至90nm。结果表明，对细胞功能的不利影响可以通过二氧化硅的表面积来确定，如通过MTT分析测得的代谢活性降低和使用活/死染色法观察到的膜通透性增加。具有最大表面积的顺铂官能化二氧化硅纳米粒子引起了最大的响应，这几乎与游离顺铂诱导的结果相同。此外，如果经过进一步的研究验证，则表明顺铂与二氧化硅纳米颗粒连接可能被证明可用于定点药物递送。