Luminescent bacteria in the family Vibrionaceae (Bacteria: γ‐Proteobacteria) are commonly found in complex, bilobed light organs of sepiolid and loliginid squids. Although morphology of these organs in both families of squid is similar, the species of bacteria that inhabit each host has yet to be verified. We utilized sequences of 16S ribosomal RNA, luciferase α‐subunit (luxA) and the glyceraldehyde‐3‐phosphate dehydrogenase (gapA) genes to determine phylogenetic relationships between 63 strains of Vibrio bacteria, which included representatives from different environments as well as unidentified luminescent isolates from loliginid and sepiolid squid from Thailand. A combined phylogenetic analysis was used including biochemical data such as carbon use, growth and luminescence. Results demonstrated that certain symbiotic Thai isolates found in the same geographic area were included in a clade containing bacterial species phenotypically suitable to colonize light organs. Moreover, multiple strains isolated from a single squid host were identified as more than one bacteria species in our phylogeny. This research presents evidence of species of luminescent bacteria that have not been previously described as symbiotic strains colonizing light organs of Indo‐West Pacific loliginid and sepiolid squids, and supports the hypothesis of a non‐species‐specific association between certain sepiolid and loliginid squids and marine luminescent bacteria.

中文翻译：

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Uroteuthis、Loliolus 和 Euprymna（软体动物：Cephalopoda）乌贼属的发光共生体之间的生物多样性

弧菌科中的发光细菌（细菌：γ-变形杆菌）通常存在于海螺科和 loliginid 鱿鱼复杂的双叶光器官中。尽管两个鱿鱼科的这些器官的形态相似，但栖息在每个宿主中的细菌种类还有待验证。我们利用 16S 核糖体 RNA、荧光素酶 α-亚基 (luxA) 和 3-磷酸甘油醛脱氢酶 (gapA) 基因的序列来确定 63 株弧菌之间的系统发育关系，其中包括来自不同环境的代表以及未鉴定的发光分离株来自泰国的 loliginid 和 sepiolid 鱿鱼。使用组合系统发育分析，包括生化数据，例如碳使用、生长和发光。结果表明，在同一地理区域发现的某些共生泰国分离株包含在一个进化枝中，该进化枝含有表型适合定殖光器官的细菌物种。此外，从单个鱿鱼宿主中分离出的多种菌株在我们的系统发育中被鉴定为不止一种细菌物种。这项研究提供了之前未被描述为共生菌株的发光细菌物种的证据，这些细菌在印度-西太平洋海螺科和海螺科鱿鱼的光器官中定居，并支持某些海螺科和海螺科鱿鱼之间存在非物种特异性关联的假设和海洋发光细菌。在我们的系统发育中，从单个鱿鱼宿主中分离出的多个菌株被鉴定为不止一种细菌物种。这项研究提供了之前未被描述为共生菌株的发光细菌物种的证据，这些细菌在印度-西太平洋海螺科和海螺科鱿鱼的光器官中定居，并支持某些海螺科和海螺科鱿鱼之间存在非物种特异性关联的假设和海洋发光细菌。在我们的系统发育中，从单个鱿鱼宿主中分离出的多种菌株被鉴定为不止一种细菌物种。这项研究提供了之前未被描述为共生菌株的发光细菌物种的证据，这些细菌在印度-西太平洋海螺科和海螺科鱿鱼的光器官中定居，并支持某些海螺科和海螺科鱿鱼之间存在非物种特异性关联的假设和海洋发光细菌。