Objective: This study aims to investigate the mechanism of proliferation potential-related protein (PP-RP) in influencing the proliferation, migration, and apoptosis of esophageal cancer cells. Methods: Quantitative real-time PCR and western blotting were performed to analyze the expression of PP-RP gene, p53, and interleukin (IL)-17 in human normal tissues and tumor tissues, as well as the expression of p53 and IL-17 in Eca109 and TE3 cells. The esophageal cancer cell proliferation was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), cell apoptosis was detected by flow cytometry, and cell migration was detected by transwell migration. Results: PP-RP expressed highly in tumor tissue and Eca109 and TE3 cells, PP-RP overexpression inhibited the expression of p53 and promoted the expression of IL-17 in Eca109 and TE3 cells. PP-RP overexpression increased the expression of F-actin, promoted cell proliferation, and migration and suppressed cell apoptosis. Cell proliferation ability and cell migration ability were significantly strengthened while apoptosis was suppressed by PP-RP + pyruvate carboxylase deoxyribonucleic acid (PCDNA)-p53 group and PP-RP + IL-17 siRNA group in TE3 cells. Conclusion: Our data suggest that PP-RP promotes esophageal cancer cell proliferation and migration, and suppresses apoptosis by mediating the expression of p53 and IL-17.

中文翻译：

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增殖潜能相关蛋白通过介导p53和白细胞介素17的表达促进食管癌细胞增殖、迁移和抑制细胞凋亡

目的：本研究旨在探讨增殖电位相关蛋白（PP-RP）影响食管癌细胞增殖、迁移和凋亡的机制。方法：采用实时荧光定量 PCR 和蛋白质印迹法分析 PP-RP 基因、p53 和白细胞介素 (IL)-17 在人正常组织和肿瘤组织中的表达，以及 p53 和 IL-17 的表达。在 Eca109 和 TE3 细胞中。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)检测食管癌细胞增殖，流式细胞仪检测细胞凋亡，Transwell迁移检测细胞迁移。结果：PP-RP在肿瘤组织和Eca109、TE3细胞中高表达，PP-RP过表达抑制了Eca109和TE3细胞中p53的表达并促进了IL-17的表达。PP-RP过表达增加F-肌动蛋白的表达，促进细胞增殖和迁移并抑制细胞凋亡。PP-RP+丙酮酸羧化酶脱氧核糖核酸(PCDNA)-p53组和PP-RP+IL-17 siRNA组在TE3细胞中显着增强细胞增殖能力和细胞迁移能力，同时抑制细胞凋亡。结论：我们的数据表明，PP-RP 通过介导 p53 和 IL-17 的表达促进食管癌细胞增殖和迁移，并抑制细胞凋亡。PP-RP+丙酮酸羧化酶脱氧核糖核酸(PCDNA)-p53组和PP-RP+IL-17 siRNA组在TE3细胞中显着增强细胞增殖能力和细胞迁移能力，同时抑制细胞凋亡。结论：我们的数据表明，PP-RP 通过介导 p53 和 IL-17 的表达促进食管癌细胞增殖和迁移，并抑制细胞凋亡。PP-RP+丙酮酸羧化酶脱氧核糖核酸(PCDNA)-p53组和PP-RP+IL-17 siRNA组在TE3细胞中显着增强细胞增殖能力和细胞迁移能力，同时抑制细胞凋亡。结论：我们的数据表明，PP-RP 通过介导 p53 和 IL-17 的表达促进食管癌细胞增殖和迁移，并抑制细胞凋亡。