Cultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or a lack of proliferation in a concentration-dependent manner. This inhibition of proliferation is accompanied by the generation of polyploid giant cancer cells (PGCCs) that are characterized by cell flattening, increased cell size, polyploidy, and polynucleation as determined by crystal violet staining, BrdU and DiI labelling, and flow cytometry as well as video time-lapse analysis. Continuous SSP treatment of A549 cells can preserve PGCCs for at least two months in a resting state. Upon removal of SSP, A549 PGCCs restart to divide and exhibit a proliferation pattern and cellular morphology indistinguishable from cells where PGCCs originally derived from. Thus, SSP-treated A549 cells represent a simple and reliable experimental model for the reversible generation of PGCCs and their subsequent experimental analysis.

中文翻译：

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星形孢菌素诱导非小细胞肺癌A549细胞中多倍体巨癌细胞的产生。

在存在星形孢菌素（SSP）的情况下培养A549非小细胞肺癌（NSCLC）细胞会导致浓度依赖性的增殖减少或缺乏。这种增殖抑制作用伴随着多倍体巨癌细胞（PGCC）的产生，其特征是细胞变平，细胞大小增加，多倍体和多核化（通过结晶紫染色，BrdU和DiI标记以及流式细胞仪确定）视频延时分析。连续SSP处理A549细胞可使PGCC在静止状态下保存至少两个月。除去SSP后，A549 PGCC重新开始分裂，并显示出与PGCC最初来源的细胞没有区别的增殖模式和细胞形态。从而，