OBJECTIVE Chikungunya virus (CHIKV) is a rapidly emerging arbovirus causing millions of infections in more than 40 countries. CHIKV is typically a biosafety level 3 pathogen in many countries and handling of CHIKV requires a high standard of laboratory safety settings. Many studies require the whole virus to be handled in a biosafety level 2 setting. A potential solution for managing this problem is pathogen inactivation without affecting its antigenicity. In the present study, we attempted to inactivate CHIKV by ultraviolet (UV) irradiation. METHODS Different UV doses were used to inactivate CHIKV. The replication status of the inactivated virus was verified in cell lines. Western blot, electron microscopy, and immune fluorescence assay were used, respectively, to view the antigenicity, structural integrity, and entry of the virus into cell lines. RESULTS The inactivation was complete when a UV dose of 0.09 J/cm2 for 3 × 30 s was used and no change in antigenicity and integrity was observed. CONCLUSIONS The study concludes that the UV-inactivated virus is antigenically stable and could be used in biosafety level 2 settings for different experiments.

中文翻译：

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基孔肯雅病毒的紫外线灭活。

目的基孔肯雅病毒（CHIKV）是一种快速出现的虫媒病毒，在40多个国家/地区引起数百万感染。CHIKV在许多国家/地区通常是3级生物安全性病原体，对CHIKV的处理要求高标准的实验室安全设置。许多研究要求将整个病毒以2级的生物安全水平处理。解决该问题的潜在解决方案是灭活病原体而不影响其抗原性。在本研究中，我们试图通过紫外线（UV）灭活CHIKV。方法采用不同的紫外线剂量灭活CHIKV。在细胞系中验证了灭活病毒的复制状态。分别使用Western印迹，电子显微镜和免疫荧光分析来观察其抗原性，结构完整性，病毒进入细胞系。结果当使用紫外线剂量为0.09 J / cm2的3×30 s时，灭活完成，并且未观察到抗原性和完整性的变化。结论研究得出的结论是，紫外线灭活病毒具有抗原稳定性，可以用于不同实验的生物安全级别2设置。