当前位置:
X-MOL 学术
›
Int. J. Obesity
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Studies on insulin secretion and the pituitary insulin secretagogue beta-cell-tropin in the sand-rat (Psammomys obesus).
International Journal of Obesity ( IF 4.9 ) Pub Date : 1987-01-01 J L Morton , S J Dunmore , A Beloff-Chain , J H Adler
International Journal of Obesity ( IF 4.9 ) Pub Date : 1987-01-01 J L Morton , S J Dunmore , A Beloff-Chain , J H Adler
The sand-rat (Psammomys obesus) is an animal model for the study of human maturity onset diabetes which appears to be controlled by caloric intake. In the present investigations, these animals have been studied in relation to the influence of low- and high-energy diets on body weight, plasma insulin and blood glucose levels, and on insulin secretion from the perfused pancreas and the secretion of corticotropin-like intermediate lobe peptide (CLIP, ACTH18-39) and the insulin secretagogue beta-cell-tropin (beta-CT, ACTH22-39) from the pituitary neurointermediate lobe. The sand-rats maintained on the high-energy diet all became obese. Insulin secretion from the perfused pancreas of the obese sand-rat in the presence of 5.6 mM glucose was significantly higher than in the lean controls maintained on low-energy diets. Increasing the glucose concentration to 16.7 mM only produced a small stimulation of insulin secretion in the obese animals, and the difference between the two groups was not significant. Stimulation of insulin secretion by beta-CT was variable, but the obese animals appeared to be more responsive. Pituitary neurointermediate lobes were incubated for 4 h to measure the secretion of the ACTH related peptide. These were separated by gel filtration and the concentrations measured by radioimmunoassay with a CLIP antiserum and a CLIP standard. In all experiments beta-CT was 4-6 per cent of the total CLIP immunoreactive material. In these experiments the obese animals maintained on a high-energy diet were divided into two groups, those with plasma insulin levels less than 500 mu u/ml and those with insulin levels greater than 500 mu u/ml. The latter group had a significantly higher blood glucose level, presumably due to the insulin resistance resulting from the severe hyperinsulinaemia. It was also observed that CLIP-IRM and beta-CT secretion was lower in this group than in the animals maintained on low-energy diets or those on high-energy diets with moderate hyperinsulinaemia. This suggests a possible feedback inhibition by insulin on the secretion of beta-CT.
中文翻译:
沙鼠(Psammomys obesus)中胰岛素分泌和垂体胰岛素促分泌素β细胞肌钙蛋白的研究。
沙鼠(Psammomys obesus)是用于研究人类成熟期糖尿病的动物模型,该糖尿病似乎受热量摄入控制。在本研究中,已对这些动物进行了研究,涉及低能量和高能量饮食对体重,血浆胰岛素和血糖水平的影响,以及灌注胰腺的胰岛素分泌和促肾上腺皮质激素样中间产物的分泌垂体神经中枢叶的肝叶肽(CLIP,ACTH18-39)和胰岛素促分泌素β-细胞肌钙蛋白(β-CT,ACTH22-39)。维持高能量饮食的沙鼠变得肥胖。在存在5.6 mM葡萄糖的情况下,肥胖沙鼠的灌注胰腺的胰岛素分泌显着高于低能量饮食所维持的瘦肉对照组。将葡萄糖浓度增加至16.7 mM只会对肥胖动物产生少量的胰岛素分泌刺激作用,并且两组之间的差异并不显着。β-CT对胰岛素分泌的刺激是可变的,但肥胖动物似乎反应更灵敏。垂体神经中间叶温育4小时以测量ACTH相关肽的分泌。通过凝胶过滤将其分离,并通过CLIP抗血清和CLIP标准品通过放射免疫测定法测量浓度。在所有实验中,β-CT占CLIP免疫反应物质总数的4-6%。在这些实验中,维持高能量饮食的肥胖动物分为两组,即血浆胰岛素水平低于500μu/ ml的动物和胰岛素水平高于500μu/ ml的动物。后者的血糖水平明显较高,可能是由于严重的高胰岛素血症引起的胰岛素抵抗所致。还观察到,与低能量饮食或中度高胰岛素血症高能量饮食维持的动物相比,该组CLIP-IRM和β-CT的分泌较低。这表明胰岛素可能会抑制β-CT的分泌。
更新日期:2019-11-01
中文翻译:
沙鼠(Psammomys obesus)中胰岛素分泌和垂体胰岛素促分泌素β细胞肌钙蛋白的研究。
沙鼠(Psammomys obesus)是用于研究人类成熟期糖尿病的动物模型,该糖尿病似乎受热量摄入控制。在本研究中,已对这些动物进行了研究,涉及低能量和高能量饮食对体重,血浆胰岛素和血糖水平的影响,以及灌注胰腺的胰岛素分泌和促肾上腺皮质激素样中间产物的分泌垂体神经中枢叶的肝叶肽(CLIP,ACTH18-39)和胰岛素促分泌素β-细胞肌钙蛋白(β-CT,ACTH22-39)。维持高能量饮食的沙鼠变得肥胖。在存在5.6 mM葡萄糖的情况下,肥胖沙鼠的灌注胰腺的胰岛素分泌显着高于低能量饮食所维持的瘦肉对照组。将葡萄糖浓度增加至16.7 mM只会对肥胖动物产生少量的胰岛素分泌刺激作用,并且两组之间的差异并不显着。β-CT对胰岛素分泌的刺激是可变的,但肥胖动物似乎反应更灵敏。垂体神经中间叶温育4小时以测量ACTH相关肽的分泌。通过凝胶过滤将其分离,并通过CLIP抗血清和CLIP标准品通过放射免疫测定法测量浓度。在所有实验中,β-CT占CLIP免疫反应物质总数的4-6%。在这些实验中,维持高能量饮食的肥胖动物分为两组,即血浆胰岛素水平低于500μu/ ml的动物和胰岛素水平高于500μu/ ml的动物。后者的血糖水平明显较高,可能是由于严重的高胰岛素血症引起的胰岛素抵抗所致。还观察到,与低能量饮食或中度高胰岛素血症高能量饮食维持的动物相比,该组CLIP-IRM和β-CT的分泌较低。这表明胰岛素可能会抑制β-CT的分泌。