Objectives: A number of studies have suggested that acrolein-induced lung injury and pulmonary diseases are associated with the depletion of antioxidants and the production of reactive oxygen species. Therefore, compounds that scavenge reactive oxygen species may exert protective effects against acrolein-induced apoptosis. Because hesperetin, a natural flavonoid, has been reported to have an antioxidant activity, we investigated the effect of hesperitin against acrolein-induced apoptosis of lung cells.

Methods: We evaluated the protective role of hesperetin in acrolein-induced lung injury using Lewis lung carcinoma (LLC) cells and mice.

Results: Upon exposure of LLC cells and mice to acrolein, hesperetin ameliorated the lung inbjury through attenuation of oxidative stress.

Conclusion: In the present report, we demonstrate that hesperetin exhibits a protective effect against acrolein-induced apoptosis of lung cells in both in vitro and in vivo models. Our study provides a useful model to investigate the potential application of hesperetin for the prevention of lung diseases associated with acrolein toxicity.

目的：大量研究表明，丙烯醛诱导的肺损伤和肺部疾病与抗氧化剂的消耗和活性氧的产生有关。因此，清除活性氧物种的化合物可能对丙烯醛诱导的细胞凋亡发挥保护作用。由于橙皮素是一种天然的类黄酮，据报道具有抗氧化活性，因此我们研究了橙皮素对丙烯醛诱导的肺细胞凋亡的影响。

方法：我们使用路易斯肺癌（LLC）细胞和小鼠评估了橙皮素在丙烯醛诱导的肺损伤中的保护作用。

结果：将LLC细胞和小鼠暴露于丙烯醛后，橙皮素可通过减轻氧化应激减轻肺损伤。

结论：在本报告中，我们证明了橙皮素在体外和体内模型中均具有抗丙烯醛诱导的肺细胞凋亡的保护作用。我们的研究提供了一个有用的模型，以研究橙皮素在预防与丙烯醛毒性有关的肺部疾病中的潜在应用。