Welding fumes contain metals and other toxic substances known or strongly suspected to be related with oxidative stress and premature cellular senescence. Apolipoprotein J/Clusterin (ApoJ/CLU) is a glycoprotein that is differentially regulated in various physiological and disease states including ageing and age-related diseases. In vitro data showed that exposure of human diploid fibroblasts to hexavalent chromium (Cr(VI)) resulted in premature senescence and significant upregulation of the ApoJ/CLU protein. In this study we analyzed blood and urine samples from shipyard industry welders being exposed to different levels of Cr(VI) over a period of five months in order to assay in vivo the relation of ApoJ/CLU serum levels with Cr(VI). Our findings confirmed the previously reported in vitro data since reduction of Cr levels, after a worksite intervention, associated with lower levels of ApoJ/CLU serum levels. We concluded that the human ApoJ/CLU gene is responsive to the acute in vivo oxidative stress induced by heavy metals such as hexavalent chromium.

中文翻译：

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焊工中六价铬的生物监测和衰老生物标志物载脂蛋白 J/Clusterin 的血清水平。

焊接烟雾含有金属和其他已知或强烈怀疑与氧化应激和细胞过早衰老有关的有毒物质。载脂蛋白 J/Clusterin (ApoJ/CLU) 是一种糖蛋白，在各种生理和疾病状态（包括衰老和年龄相关疾病）中受到差异调节。体外数据显示，人类二倍体成纤维细胞暴露于六价铬 (Cr(VI)) 会导致过早衰老和 ApoJ/CLU 蛋白的显着上调。在这项研究中，我们分析了在五个月内暴露于不同水平的 Cr(VI) 的造船业焊工的血液和尿液样本，以在体内测定 ApoJ/CLU 血清水平与 Cr(VI) 的关系。我们的研究结果证实了先前报告的体外数据，因为 Cr 水平降低，工地干预后，与较低水平的 ApoJ/CLU 血清水平相关。我们得出结论，人类 ApoJ/CLU 基因对重金属（如六价铬）诱导的体内急性氧化应激有反应。