Considerable interest has recently mounted regarding the biological roles of Gram-negative outer membrane vesicles (MVs). The first discovery of MVs was made over four decades ago, and it is now clear that most Gram-negative bacteria produce MVs, with Pseudomonas aeruginosa and Escherichia coli as the most extensively studied. Much of our knowledge of the biological roles of MVs and mechanism of MV formation is due to T.J. Beveridge and colleagues. Beveridge pioneered the field of MV research not only by enhancing our understanding of MV function, but also through the application of a wide variety of physical, chemical, and genetic techniques to complement his elegant electron microscopy investigations. Here we review the contributions of Beveridge's group to our understanding of MV biology.

中文翻译：

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革兰氏阴性外膜囊泡：超出细胞表面。

最近人们对革兰氏阴性外膜囊泡 (MV) 的生物学作用产生了相当大的兴趣。MVs 的首次发现是在 40 多年前进行的，现在很明显，大多数革兰氏阴性菌都会产生 MVs，其中绿脓杆菌和大肠杆菌是研究最广泛的。我们对 MV 的生物学作用和 MV 形成机制的大部分了解归功于 TJ Beveridge 及其同事。Beveridge 开创了 MV 研究领域，不仅通过增强我们对 MV 功能的理解，而且还通过应用各种物理、化学和遗传技术来补充他优雅的电子显微镜研究。在这里，我们回顾了 Beveridge 小组对我们理解 MV 生物学的贡献。