The use of plants for production of recombinant proteins is becoming widely accepted. More recently, plant cell cultures have been proposed as valuable systems for producing a wide range of biologically active proteins. Such systems provide certain advantages over whole plants, but yields are still considered a limitation. In this study we established a Medicago truncatula cell suspension line expressing phytase from Aspergillus niger. Phytase is an N-glycosylated enzyme that breaks down indigestible phytate, resulting in an increased availability of phosphorus and other minerals in monogastric animals and reduced levels of phosphorus output in their manure. Various production systems have previously been used to express heterologous phytase, including several plant species. In this work, remarkable amounts of enzymatically active recombinant phytase were produced and secreted into the culture medium. Recombinant phytase accumulated to at least 25 mg/L and remained stable along the growth curve, and an enriched fraction with high enzymatic activity was easily obtained. We therefore propose M. truncatula cell suspension cultures as a potential system for the production of recombinant proteins. Most importantly, we have shown that, contrary to general belief, it is possible to achieve high levels of a functional recombinant protein in plant cell culture systems.

中文翻译：

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高水平的稳定肌醇六磷酸酶在转基因苜蓿苜蓿细胞悬浮培养物的培养基中积累。

使用植物生产重组蛋白已被广泛接受。最近，已经提出了植物细胞培养物作为产生广泛的生物活性蛋白的有价值的系统。这样的系统相对于整个植物具有某些优势，但是产量仍然被认为是限制。在这项研究中，我们建立了一个表达来自黑曲霉的植酸酶的紫花苜蓿细胞悬浮株。植酸酶是一种N-糖基化酶，可以分解难以消化的肌醇六磷酸，导致单胃动物中磷和其他矿物质的利用率增加，粪便中磷的输出水平降低。先前已使用各种生产系统来表达异源植酸酶，包括几种植物。在这项工作中 产生了大量酶活性的重组肌醇六磷酸酶，并分泌到培养基中。重组肌醇六磷酸酶积累到至少25 mg / L，并沿生长曲线保持稳定，并且容易获得具有高酶活性的富集级分。因此，我们提出了截枝分枝杆菌细胞悬浮培养物作为生产重组蛋白的潜在系统。最重要的是，我们已经证明，与普遍的看法相反，有可能在植物细胞培养系统中实现高水平的功能重组蛋白。truncatula细胞悬浮培养物是生产重组蛋白的潜在系统。最重要的是，我们已经证明，与普遍的看法相反，有可能在植物细胞培养系统中实现高水平的功能重组蛋白。truncatula细胞悬浮培养物是生产重组蛋白的潜在系统。最重要的是，我们已经证明，与普遍的看法相反，有可能在植物细胞培养系统中实现高水平的功能重组蛋白。