A bacterial strain using pyridine as sole carbon, nitrogen and energy source was isolated from the activated sludge of a coking wastewater treatment plant. By means of morphologic observation, physiological characteristics study and 16S rRNA gene sequence analysis, the strain was identified as the species of Paracoccus. The strain could degrade 2,614 mg l(-1) of pyridine completely within 49.5 h. Experiment designed to track the metabolic pathway showed that pyridine ring was cleaved between the C2 and N, then the mineralization of the carbonous intermediate products may comply with the early proposed pathway and the transformation of the nitrogen may proceed on a new pathway of simultaneous heterotrophic nitrification and aerobic denitrification. During the degradation, NH3-N occurred and increased along with the decrease of pyridine in the solution; but the total nitrogen decreased steadily and equaled to the quantity of NH3-N when pyridine was degraded completely. Adding glucose into the medium as the extra carbon source would expedite the biodegradation of pyridine and the transformation of the nitrogen. The fragments of nirS gene and nosZ gene were amplified which implied that the BW001 had the potential abilities to reduce NO2- to NO and/or N2O, and then to N2.

中文翻译：

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副球菌对微生物的降解和吡啶的代谢途径。菌株BW001。

从焦化废水处理厂的活性污泥中分离出使用吡啶作为唯一碳，氮和能源的细菌菌株。通过形态观察，生理特性研究和16S rRNA基因序列分析，确定该菌株为副球菌。该菌株可在49.5小时内完全降解2,614 mg l（-1）吡啶。跟踪代谢途径的实验表明，吡啶环在C2和N之间断裂，然后碳质中间产物的矿化可能符合早期提出的途径，而氮的转化可能在同时异养硝化的新途径上进行和有氧反硝化。在退化期间，随着溶液中吡啶的减少，NH3-N发生并增加。但是当吡啶完全降解时，总氮稳定下降，等于NH3-N的量。将葡萄糖添加到培养基中作为额外的碳源将加速吡啶的生物降解和氮的转化。扩增了nirS基因和nosZ基因的片段，这表明BW001具有将NO 2-还原为NO和/或N 2 O然后还原为N 2的潜在能力。