Imaging membrane dynamics is an important goal, motivated by the abundance of biochemical and biophysical events that are orchestrated at, or by, cellular membranes. The short length scales, fast timescales, and environmental requirements of membrane phenomena present challenges to imaging experiments. Several technical advances offer means to overcome these challenges, and we describe here three powerful techniques applicable to membrane imaging: total internal reflection fluorescence (TIRF) microscopy, fluorescence interference contrast (FLIC) microscopy, and fluorescence correlation spectroscopy (FCS). For each, we discuss the physics underpinning the approach, its practical implementation, and recent examples highlighting its achievements in exploring the membrane environment.

中文翻译：

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膜动力学的荧光成像。

对膜动力学进行成像是一个重要的目标，这是由在细胞膜或细胞膜上精心安排的大量生化和生物物理事件所激发的。短长度尺度，快速时间尺度和膜现象的环境要求对成像实验提出了挑战。多项技术进步为克服这些挑战提供了手段，我们在此介绍适用于膜成像的三种强大技术：全内反射荧光（TIRF）显微镜，荧光干涉对比（FLIC）显微镜和荧光相关光谱（FCS）。对于每种方法，我们都讨论了支持该方法的物理原理，其实际实现方式，以及最近的一些实例，突出了该方法在探索膜环境方面的成就。