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Microbial degradation of 7-ketocholesterol.
Biodegradation ( IF 3.6 ) Pub Date : 2008-03-18 , DOI: 10.1007/s10532-008-9183-5
Jacques Mathieu 1 , John Schloendorn , Bruce E Rittmann , Pedro J J Alvarez
Affiliation  

7-Ketocholesterol (7KC) is an oxidized derivative of cholesterol suspected to be involved in the pathogenesis of atherosclerosis and possibly Alzheimer's disease. While some oxysterols are important biological mediators, 7KC is generally cytotoxic and interferes with cellular homeostasis. Despite recent interest in preventing the accumulation of 7KC in a variety of matrices to avoid adverse biological effects, its microbial degradation has not been previously addressed in the peer-reviewed literature. Thus, the rate and extent of biodegradation of this oxysterol was investigated to bridge this gap. A wide variety of bacteria isolated from soil or activated sludge, including Proteobacterium Y-134, Sphingomonas sp. JEM-1, Nocardia nova, Rhodococcus sp. RHA1, and Pseduomonas aeruginosa, utilized 7KC as a sole carbon and energy source, resulting in its mineralization. Nocardia nova, which is known to produce biosurfactants, was the fastest degrader. This study supports the notion that microbial catabolic enzymes could be exploited to control 7KC levels in potential biotechnological applications for agricultural, environmental, or medical use.

中文翻译:

7-酮胆固醇的微生物降解。

7-Ketocholesterol(7KC)是胆固醇的一种氧化衍生物,被怀疑与动脉粥样硬化和阿尔茨海默氏病的发病有关。虽然某些氧固醇是重要的生物介质,但7KC通常具有细胞毒性,并会干扰细胞体内稳态。尽管最近有兴趣防止7KC在各种基质中积累以避免不利的生物学影响,但其微生物降解以前尚未在同行评审的文献中得到解决。因此,研究了该氧固醇的生物降解速率和程度以弥合这一差距。从土壤或活性污泥中分离出的细菌种类繁多,包括变形杆菌Y-134,鞘氨醇单胞菌(Sphingomonas sp。)。JEM-1,诺卡氏菌新星,红球菌 RHA1和铜绿假单胞菌利用7KC作为唯一的碳和能源,导致其矿化。已知会产生生物表面活性剂的诺卡氏菌新星是最快的降解剂。这项研究支持以下观点:在农业,环境或医学用途的潜在生物技术应用中,可利用微生物分解代谢酶来控制7KC水平。
更新日期:2019-11-01
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