Anaerobic bacteria have been used to produce 2,4-dihydroxylamino-nitrotoluene (2,4DHANT), a reductive metabolite of 2,4,6-trinitrotoluene (TNT). Here, an aerobic TNT biodegrader Pseudomonas sp. strain TM15 produced 2,4DHANT as evidenced by the molecular ion with m/z of 199 identified from LC-TOFMS analyses. TNT biodegradation with a high cell concentration (10(9) cells/ml) led to a significant accumulation of 2,4DHANT in the culture medium, as well as hydroxylamino-dinitrotoluenes (HADNTs), although these products were not accumulated when a low cell concentration was used; also, the accumulation of diamino-nitrotoluene and of an unidentified metabolite were observed in the culture medium with the high cell concentration (10(10) cells/ml). 2,4DHANT overproduction was a function of the aeration speed since cultures with low aeration speeds (30 rpm) had a 19-fold higher DHANT productivity than those aerated with high speeds (180 rpm); this indicates that molecular oxygen was related to the formation of 2,4DHANT. The quantification of dissolved oxygen (DO) in the media demonstrated that the productivity of 2,4DHANT was increased at low DO values. Moreover, supplying oxygen to the culture media produced a remarkable decrease of 2,4DHANT accumulation; these results clearly indicate that high 2,4DHANT production was a consequence of the oxygen deficit in the culture medium. This finding is useful for understanding the TNT biodegradation (bioremediation technology) in an anoxic environment.

中文翻译：

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好氧TNT降解菌假单胞菌（Pseudomonas sp。）对TNT的生物降解和生产二羟基氨基-硝基甲苯。缺氧环境中的菌株TM15。

厌氧细菌已被用于生产2,4,6-三硝基甲苯（TNT）的还原性代谢产物2,4-二羟基氨基-硝基甲苯（2,4DHANT）。在这里，有氧TNT生物降解假单胞菌。由LC-TOFMS分析鉴定出的m / z为199的分子离子证明了TM15菌株产生了2,4DHANT。高细胞浓度（10（9）细胞/ ml）的TNT生物降解导致2,4DHANT和羟氨基二硝基甲苯（HADNT）在培养基中大量积聚，尽管这些产物在低细胞时不会积聚。使用浓度；同样，在高细胞浓度（10（10）细胞/ ml）的培养基中观察到二氨基硝基甲苯和未知代谢物的积累。2，4DHANT过量生产是通气速度的函数，因为低通气速度（30 rpm）的培养物的DHANT生产率比高速通气（180 rpm）的高19倍；这表明分子氧与2,4DHANT的形成有关。介质中溶解氧（DO）的定量表明，在低DO值下2,4DHANT的生产率提高了。而且，向培养基供应氧气显着减少了2,4DHANT的积累。这些结果清楚地表明，高2,4DHANT产生是培养基中氧缺乏的结果。该发现对于理解缺氧环境中的TNT生物降解（生物修复技术）很有用。这表明分子氧与2,4DHANT的形成有关。介质中溶解氧（DO）的定量表明，在低DO值下2,4DHANT的生产率提高了。而且，向培养基供应氧气显着减少了2,4DHANT的积累。这些结果清楚地表明，高2,4DHANT产生是培养基中氧缺乏的结果。该发现对于理解缺氧环境中的TNT生物降解（生物修复技术）很有用。这表明分子氧与2,4DHANT的形成有关。介质中溶解氧（DO）的定量表明，在低DO值下2,4DHANT的生产率提高了。而且，向培养基供应氧气显着减少了2,4DHANT的积累。这些结果清楚地表明，高2,4DHANT产生是培养基中氧缺乏的结果。该发现对于理解缺氧环境中的TNT生物降解（生物修复技术）很有用。4DHANT积累；这些结果清楚地表明，高2,4DHANT产生是培养基中氧缺乏的结果。该发现对于理解缺氧环境中的TNT生物降解（生物修复技术）很有用。4DHANT积累；这些结果清楚地表明，高2,4DHANT产生是培养基中氧缺乏的结果。该发现对于理解缺氧环境中的TNT生物降解（生物修复技术）很有用。