The biotransformation of phenazine-1-carboxylic acid (PCA) by PCA-degrading strain Sphingomonas sp. DP58 yielded small quantities of metabolites and was demonstrated for the first time. The metabolites were isolated by using preparative high-performance liquid chromatography (HPLC). In addition, these were subsequently characterized by gas chromatography (GC)-mass spectrum (MS) after N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) derivatization and (1)H-nuclear magnetic resonance (NMR). They were identified as 4-hydroxy-1-(2-carboxyphenyl) azacyclobut-2-ene-2-carbonitrile (HPAEC) and 4-hydroxy-1-(2-carboxyphenyl)-2-azetidinecarbonitrile (HPAC). The two metabolites had transformational relationship between each other.

中文翻译：

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Sphingomonas sp。菌株对吩嗪-1-羧酸的代谢降解。DP58：两种代谢物的鉴定。

降解PCA的Sphingomonas sp。菌株对吩嗪-1-羧酸（PCA）的生物转化。DP58产生少量代谢产物，并首次得到证明。通过使用制备型高效液相色谱（HPLC）分离代谢产物。此外，在N，O-双（三甲基甲硅烷基）三氟乙酰胺（BSTFA）衍生化和（1）H-核磁共振（NMR）之后，通过气相色谱（GC）-质谱（MS）对其进行表征。它们被鉴定为4-羟基-1-（2-羧基苯基）氮杂环丁-2-烯-2-腈（HPAEC）和4-羟基-1-（2-羧基苯基）-2-氮杂环丁烷腈（HPAC）。两种代谢物之间具有转化关系。