As several authors have reported previously, the Biodesulfurization of hydrodesulfurization recalcitrants, such as dibenzothiophene, is not yet commercially viable because mass transfer limitations and feedback inhibition effects are produced during the conversion. This work has been focused to investigate the inhibition process in aqueous and oil-water systems with two different aerobic biocatalysts types, Rhodococcus erythropolis IGTS8 and Pseudomonas putida CECT 5279. The results obtained have proven that global DBT desulfurization process using CECT 5279 was not clearly deactivated due to final product accumulation, under the experimental conditions assayed. Consistently, the desulfurization pattern has been described with the Michaelis-Menten equation, determining the kinetic parameters. On other hand, the assays have shown that important mass transfer limitations produced the decrease of the yields obtained with this Gram(-) strain in biphasic media. With strain IGTS8 it was observed lower mass transfer problems, but contrary the reaction was severely affected by the final product accumulation, in both aqueous and biphasic systems. Therefore it has been proposed an enzymatic kinetic model with competitive inhibition to describe the BDS evolution pattern when this Gram(+) strain was used.

中文翻译：

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副产物对双相介质中二苯并噻吩生物脱硫的抑制作用的描述。

如几位作者先前报道的那样，加氢脱硫顽固剂（例如二苯并噻吩）的生物脱硫在商业上尚不可行，因为在转化过程中会产生传质限制和反馈抑制作用。这项工作的重点是研究具有两种不同需氧生物催化剂类型的红球菌IGTS8和恶臭假单胞菌CECT 5279在水和油水系统中的抑制过程。获得的结果证明，使用CECT 5279的全球DBT脱硫过程并未明显失活。由于最终产物的积累，在实验条件下进行了测定。一致地，已经用Michaelis-Menten方程描述了脱硫模式，确定了动力学参数。在另一方面，分析表明，重要的传质限制导致这种Gram（-）菌株在双相介质中获得的产量下降。对于IGTS8菌株，观察到较低的传质问题，但是相反，在水性和双相体系中，反应均受到最终产物积累的严重影响。因此，已经提出了具有竞争性抑制作用的酶动力学模型来描述当使用该革兰氏（+）菌株时BDS的进化模式。