This work is focused on the evaluation of a beta-cyclodextrin polymer as a carrier medium in a fluidized bed bioreactor treating aqueous phenol as a model pollutant. The insoluble polymer support was obtained in the shape of spherical beads by crosslinking beta-cyclodextrin with epichlorohydrin. A batch of swollen polymer particles was loaded into the reactor and inoculated with a mixed bacterial culture. Bacterial growth on the polymer beads was initially stimulated by glucose addition to the medium, and then gradually replaced with phenol. The operational variables studied after the acclimation period included phenol load, hydraulic residence time and recirculation flow rate. Low hydraulic residence times and moderate phenol loads were applied. The elimination capacity was usually about 1.0 kg-phenol/m(3)d, although a maximum of 2.8 kg-phenol/m(3)d was achieved with a retention time of only 0.55 h. The depuration efficiency was not affected by the recirculation flow rate in the range studied. Neither operational nor support stability problems were detected during the operation. A high degree of expansion was achieved in the bioreactor due to the hydrogel nature of the cyclodextrin polymer and, consequently, a low energy requirement was necessary to fluidize the bed.

中文翻译：

---

以环糊精聚合物为生物膜载体的流化床生物反应器中苯酚降解的可行性研究。

这项工作的重点是在将水性苯酚作为模型污染物处理的流化床生物反应器中，作为载体介质的β-环糊精聚合物的评估。通过将β-环糊精与表氯醇交联获得球形珠状的不溶性聚合物载体。将一批溶胀的聚合物颗粒装载到反应器中，并用混合细菌培养物接种。首先通过向培养基中添加葡萄糖来刺激聚合物珠上的细菌生长，然后逐渐用苯酚代替。在适应期之后研究的操作变量包括苯酚负荷，水力停留时间和再循环流量。液压停留时间短，苯酚负荷适中。消除能力通常约为1.0 kg-酚/ m（3）d，尽管最大值为2。保留时间仅为0.55小时，达到8千克苯酚/ m（3）d。在所研究的范围内，净化效率不受再循环流速的影响。在操作过程中未发现操作或支持稳定性问题。由于环糊精聚合物的水凝胶性质，在生物反应器中实现了高度的膨胀，因此，流化床所需的能量需求较低。