This experimental study aimed to evaluate colon healing after portal ischemia followed by reperfusion. Seventy male Wistar rats randomly distributed in four groups were used: Group 1, colonic anastomosis (n = 20); Group 2, portal ischemia-reperfusion (n = 20); Group 3, colonic anastomosis and portal ischemia-reperfusion (n = 20); and Group 4, control (n = 10). In the postoperative period, these rats were re-allocated into subgroups and lipid peroxidation and protein oxidation plasma levels were evaluated on days 1 and 5 by thiobarbituric acid reactive substances (TBARS) and slot-blotting assays, respectively. A segment of the right colon was also removed for collagen analysis. Both malondialdehyde (MDA) and protein carbonyl levels (oxidative markers of lipids and proteins) presented a significant increase after reperfusion in Group 3 on days 1 (P < 0.002) and 5 (P < 0.0001). In this same group, an extensive inflammatory process showing decreased fibroplasia was observed, with deficiency in collagen deposition on both sides of the anastomosis edges. Taken together, these results indicate that portal congestion followed by reperfusion induces an oxidative stress, which impaired the mechanism of colon anastomotic healing.

中文翻译：

---

门静脉缺血和再灌注后的结肠愈合：具有氧化应激生物标志物的实验研究。

这项实验研究旨在评估门静脉缺血后再灌注后结肠的愈合。使用随机分成四组的70只雄性Wistar大鼠：第1组，结肠吻合术（n ＝ 20）；第1组。第2组，门静脉缺血再灌注（n = 20）；第三组，结肠吻合和门静脉缺血再灌注（n = 20）；第4组，控制组（n = 10）。在术后期间，将这些大鼠重新分配为亚组，并在第1天和第5天分别通过硫代巴比妥酸反应性物质（TBARS）和缝隙印迹测定法评估脂质过氧化和蛋白氧化血浆水平。还去除了右结肠的一部分以进行胶原蛋白分析。丙二醛（MDA）和蛋白质羰基水平（脂质和蛋白质的氧化标志物）在第1天（P <0.002）和第5天（P <0.0001）的第3组再灌注后均呈现显着增加。在同一组中，观察到广泛的炎症过程，显示出纤维化减少，在吻合边缘的两侧胶原沉积不足。两者合计，这些结果表明门静脉充血，然后再灌注诱导氧化应激，这削弱了结肠吻合愈合的机制。