The antioxidant effect of the ethanolic extract of Hemidesmus indicus R.Br. root (EHI), an indigenous Ayurvedic medicinal plant in India, was studied in rats with ethanol-induced nephrotoxicity. Administering 5 g/kg body weight/day of ethanol for 60 days to male Wistar rats resulted in significantly elevated levels of serum urea, creatinine and uric acid as well as kidney thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LOOH) and conjugated dienes (CD) as compared to those of the experimental control rats. Decreased levels of kidney superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), vitamin C and vitamin E were also observed on alcohol administration as compared with those of the experimental control rats. EHI was administered at a dose of 500 mg/kg body weight/day for the last 30 days of the experiment to rats with ethanol-induced kidney injury, which significantly decreased the levels of serum urea, uric acid and creatinine as well as kidney TBARS, LOOH and CD and significantly elevated the activities of SOD, CAT, GPx, GSH, vitamin C and vitamin E in kidney as compared to that of untreated ethanol-administered rats. Histopathological observations also correlated with the biochemical parameters. Thus, the data indicate that treatment with EHI offers protection against free radical-mediated oxidative stress in kidney of animals with ethanol-induced nephrotoxicity.

中文翻译：

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Hemidesmus indicus R.Br.的影响 提取物对乙醇介导的大鼠肾脏氧化损伤的作用。

棉铃虫乙醇提取物的抗氧化作用。根（EHI）是印度本土的一种印度草药草药，已在具有乙醇诱导的肾毒性的大鼠中进行了研究。对雄性Wistar大鼠施用5 g / kg体重/天的乙醇60天，导致血清尿素，肌酐和尿酸以及肾硫代巴比妥酸反应性物质（TBARS），脂质氢过氧化物（LOOH）和结合物的水平显着升高二烯（CD）与实验对照组的大鼠相比。与实验对照组相比，酒精给药后还发现肾脏超氧化物歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GPx），谷胱甘肽（GSH），维生素C和维生素E含量降低。在实验的最后30天中，EHI以500 mg / kg体重/天的剂量施用于乙醇诱发的肾损伤的大鼠，该大鼠可显着降低血清尿素，尿酸和肌酐以及肾脏TBARS的水​​平与未经乙醇处理的大鼠相比，LOOH和CD可以显着提高肾脏中SOD，CAT，GPx，GSH，维生素C和维生素E的活性。组织病理学观察还与生化参数相关。因此，数据表明，用EHI进行治疗可防止乙醇介导的肾毒性动物抵抗肾脏介导的自由基介导的氧化应激。与未经处理的乙醇大鼠相比，LOOH和CD可以显着提高肾脏中SOD，CAT，GPx，GSH，维生素C和维生素E的活性。组织病理学观察还与生化参数相关。因此，数据表明，用EHI进行治疗可防止乙醇介导的肾毒性动物抵抗肾脏介导的自由基介导的氧化应激。与未经处理的乙醇大鼠相比，LOOH和CD可以显着提高肾脏中SOD，CAT，GPx，GSH，维生素C和维生素E的活性。组织病理学观察还与生化参数相关。因此，数据表明，用EHI进行治疗可防止乙醇介导的肾毒性动物抵抗肾脏介导的自由基介导的氧化应激。