There is a wealth of new fluorescent reporter technologies for tagging of many cellular and subcellular processes in vivo. This imposed contrast is now captured with an increasing number of available imaging methods that offer new ways to visualize and quantify fluorescent markers distributed in tissues. This is an evolving field of imaging sciences that has already achieved major advances but is also facing important challenges. It is nevertheless well poised to significantly impact the ways of biological research, drug discovery, and clinical practice in the years to come. Herein, the most pertinent technologies associated with in vivo noninvasive or minimally invasive fluorescence imaging of tissues are summarized. Focus is given to small-animal imaging. However, while a broad spectrum of fluorescence reporter technologies and imaging methods are outlined, as necessary for biomedical research, and clinical translation as well.

中文翻译：

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荧光分子成像。

有大量新的荧光报告基因技术可用于标记体内许多细胞和亚细胞过程。现在，越来越多的可用成像方法捕获了这种强加的对比度，这些成像方法提供了可视化和量化组织中分布的荧光标记物的新方法。这是成像科学的一个不断发展的领域，已经取得了重大进展，但同时也面临着重大挑战。尽管如此，它已准备好在未来几年中显着影响生物学研究，药物发现和临床实践的方式。在此，总结了与组织的体内无创或微创荧光成像相关的最相关的技术。重点放在小动物成像上。然而，