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[An experimental study on destruction of K562 and HL60 induced by 5-aminolaevulinic acid-based photodynamic therapy].
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi Pub Date : 2005-07-15
Baoqin Zhang 1 , Lixia Miao , Zhenxi Zhang , Mi Xiao , Meilan Chen
Affiliation  

This experiment was designed to explore the pattern of K562 and HL60 leukemia cells death, the effects on their cell cycle and the cytoplasmic free calcium concentration ([Ca2+]i) induced by 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT). Under the transmission electron microscope (TEM), two kinds of leukemia cells' ultrastructure were observed. Flow cytometry combined with Annexin V-FITC/PI labeling was used to detect the pattern of K562 and HL60 cells' death induced by ALA-PDT. Flow cytometry combined with PI labeling was used to analyze the change in the cell cycle induced by ALA-PDT, and confocal laser scanning microscopy (CLSM) combining with calcium fluorescence probe was used to detect the change in the cytoplasmic free calcium concentration ([Ca2+]i). Immediately after irradiation, many typical apoptotic bodies were seen in the cells treated. Most of the cells treated were necrotic at 24 hours following irradiation. Flow cytometry analysis suggested that the main patterns of the cells' death were apoptosis immediately after irradiation and necrosis post-apoptosis at 24 hours post irradiation. Immediately and 24 hours after irradiation, the proportion of S phase of K562 was 57. 67% +/- 1.13% and 84.77% +/- 6.20% respectively, and the proportion of S phase of HL60 was 74.60% +/- 7.27% and 84.60% 1.74% respectively. Both [Ca+]i of the treated K562 and HL60 were increased obviously. In the best experiment condition, the initial pattern of the K562 and HL60 leukemia cells' death induced by PDT was apoptosis and the main pattern was necrosis post apoptosis. The two kinds of cells were arrested at S phase by ALA-PDT. During the death of the leukemia cells, the increase in intracellular free calcium concentration could be responsible for the ALA photodynamically induced damage to K562 and HL60 cells.

中文翻译:

基于5-氨基松油酸的光动力疗法诱导K562和HL60破坏的实验研究

本实验旨在探讨基于5-氨基松油酸的光动力疗法(ALA-PDT)诱导的K562和HL60白血病细胞死亡模式,对其细胞周期的影响以及细胞质游离钙浓度([Ca2 +] i)。在透射电镜下观察了两种白血病细胞的超微结构。流式细胞仪结合膜联蛋白V-FITC / PI标记用于检测ALA-PDT诱导的K562和HL60细胞死亡的模式。流式细胞术结合PI标记用于分析ALA-PDT诱导的细胞周期变化,共聚焦激光扫描显微镜(CLSM)与钙荧光探针结合用于检测细胞质游离钙浓度([Ca2 + ]一世)。照射后立即 在处理的细胞中观察到许多典型的凋亡小体。照射后24小时,大多数处理过的细胞坏死。流式细胞仪分析表明,细胞死亡的主要模式是照射后立即凋亡和照射后24小时细胞凋亡坏死。照射后立即和24小时,K562的S相比例分别为57. 67%+/- 1.13%和84.77%+/- 6.20%,HL60的S相比例为74.60%+/- 7.27%和84.60%1.74%。处理后的K562和HL60的[Ca +] i均明显增加。在最佳实验条件下,PDT诱导的K562和HL60白血病细胞死亡的初始模式为细胞凋亡,主要模式为细胞凋亡后坏死。两种细胞被ALA-PDT阻滞在S期。
更新日期:2019-11-01
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