Previous research has suggested that enhanced biological phosphorus removal (EBPR) from wastewater can be achieved under continuous aerobic conditions over the short term. However, little is known how environmental conditions might affect aerobic EBPR performance. Consequently we have investigated the impact of temperature, pH and dissolved oxygen (DO) concentrations on EBPR performance under strictly aerobic conditions. A sequencing batch reactor (SBR) was operated for 108 days on a six-hour cycle (four cycles a day). The SBR ran under alternating anaerobic-aerobic conditions as standard and then operated under strictly aerobic conditions for one cycle every three or four days. SBR operational temperature (10, 15, 20, 25 and 30°C), pH (6, 7, 8 and 9) and DO concentration (0.5, 2.0 and 3.5mg/L) were changed consecutively during the aerobic cycle. Recorded increases in mixed liquor phosphorus (P) concentrations during aerobic carbon source uptake (P release) were affected by the biomass P content rather than the imposed changes in the operational conditions. Thus, P release levels increased with biomass P content. By contrast, subsequent aerobic P assimilation (P uptake) levels were both affected by changes in operational temperature and pH, and peaked at 20-25°C and pH 7-8. Highest P uptake detected under these SBR operating conditions was 15.4 mg Pg-MLSS(-1) (at 25°C, pH 7 and DO 2.0mg/L). The ability of the community for linked aerobic P release and P uptake required the presence of acetate in the medium, a finding which differs from previous data, where these are reported to occur in the absence of any exogenous carbon source. Fluorescence in situ hybridization was performed on samples collected from the SBR, and Candidatus 'Accumulibacter phosphatis' cells were detected with PAOmix probes through the operational periods. Thus, Candidatus 'Accumulibacter phosphatis' seemed to perform P removal in the SBR as shown in previous studies on P removal under strictly aerobic conditions.

中文翻译：

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严格好氧条件下温度、pH 值和溶解氧浓度对强化生物除磷的影响

先前的研究表明，可以在短期内在连续有氧条件下实现废水中强化生物除磷 (EBPR)。然而，人们对环境条件如何影响有氧 EBPR 性能知之甚少。因此，我们研究了在严格有氧条件下温度、pH 值和溶解氧 (DO) 浓度对 EBPR 性能的影响。序批式反应器 (SBR) 以 6 小时循环（每天 4 个循环）运行 108 天。SBR 在交替的厌氧-好氧条件下运行作为标准，然后在严格的好氧条件下每三或四天运行一个循环。在有氧循环期间，SBR 操作温度（10、15、20、25 和 30°C）、pH（6、7、8 和 9）和溶解氧浓度（0.5、2.0 和 3.5mg/L）连续变化。在有氧碳源吸收（P 释放）期间混合液磷（P）浓度的记录增加受生物质 P 含量的影响，而不是受操作条件的强加变化的影响。因此，磷释放水平随着生物量磷含量的增加而增加。相比之下，随后的有氧 P 同化（P 吸收）水平均受操作温度和 pH 值变化的影响，并在 20-25°C 和 pH 值 7-8 时达到峰值。在这些 SBR 操作条件下检测到的最高 P 吸收量为 15.4 mg Pg-MLSS(-1)（在 25°C、pH 7 和 DO 2.0mg/L）。群落有氧 P 释放和 P 吸收相关的能力需要培养基中存在乙酸盐，这一发现与之前的数据不同，据报道，这些数据是在没有任何外源碳源的情况下发生的。对从 SBR 收集的样品进行荧光原位杂交，并在整个操作期间用 PAOmix 探针检测候选“磷酸蓄积杆菌”细胞。因此，如先前关于严格有氧条件下除磷的研究所示，Candidatus 'Accumulibacter phosphatis' 似乎在 SBR 中进行除磷。