In this study, we report the fabrication of engineered iron oxide magnetic nanoparticles (MNPs) functionalized with anti-human epidermal growth factor receptor type 2 (HER2) antibody to target the tumor antigen HER2. The Fc-directed conjugation of antibodies to the MNPs aids their efficient immunospecific targeting through free Fab portions. The directional specificity of conjugation was verified on a macrophage cell line. Immunofluorescence studies on macrophages treated with functionalized MNPs and free anti-HER2 antibody revealed that the antibody molecules bind to the MNPs predominantly through their Fc portion. Different cell lines with different HER2 expression levels were used to test the specificity of our functionalized nanoprobe for molecular targeting applications. The results of cell line targeting demonstrate that these engineered MNPs are able to differentiate between cell lines with different levels of HER2 expression.

中文翻译：

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用于增强分子靶向的磁性纳米粒子的 Fc 导向抗体偶联

在这项研究中，我们报告了用抗人表皮生长因子受体 2 (HER2) 抗体功能化的工程化氧化铁磁性纳米粒子 (MNP) 的制造，以靶向肿瘤抗原 HER2。抗体与 MNP 的 Fc 定向缀合有助于它们通过游离 Fab 部分进行有效的免疫特异性靶向。在巨噬细胞系上验证了缀合的方向特异性。对用功能化 MNP 和游离抗 HER2 抗体处理的巨噬细胞的免疫荧光研究表明，抗体分子主要通过其 Fc 部分与 MNP 结合。使用具有不同 HER2 表达水平的不同细胞系来测试我们的功能化纳米探针在分子靶向应用中的特异性。