In this paper, we describe a graft polymerization/solvent immersion method for generating poly(2-hydroxyethyl methacrylate) (PHEMA) brushes in various patterns. We used a novel fabrication process, involving very-large-scale integration and oxygen plasma treatment, to generate well-defined patterns of polymerized PHEMA on patterned Si(100) surfaces. We observed brush- and mushroom-like regions for the PHEMA brushes, with various pattern resolutions, after immersing wafers presenting lines of these polymers in MeOH and n-hexane, respectively. The interaction between PHEMA and ferritin protein sheaths in MeOH and n-hexane (good and poor solvent for PHEMA, respectively) was used to capture and release ferritins from fluidic system. The “tentacles” behaver for PHEMA brushes was found through various solvents in fluidic system. Using high-resolution scanning electron microscopy, we observed patterned ferritin Fe cores on the Si surface after pyrolysis of the patterned PHEMA brushes and ferritin protein sheaths, which verify the “tentacles” behaver for PHEMA brushes.

中文翻译：

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铁蛋白固定在来自胶体系统的硅表面上的图案化聚（甲基丙烯酸 2-羟乙酯）刷上

在本文中，我们描述了一种用于生成各种图案的聚（2-羟乙基甲基丙烯酸酯）（PHEMA）刷子的接枝聚合/溶剂浸渍方法。我们使用了一种新颖的制造工艺，包括非常大规模的集成和氧等离子体处理，在图案化的 Si(100) 表面上生成定义明确的聚合 PHEMA 图案。在将呈现这些聚合物线的晶片分别浸入甲醇和正己烷后，我们观察到 PHEMA 刷子的刷子和蘑菇状区域，具有不同的图案分辨率。PHEMA 与 MeOH 和正己烷（分别是 PHEMA 的良溶剂和不良溶剂）中的铁蛋白鞘之间的相互作用用于从流体系统中捕获和释放铁蛋白。PHEMA 刷子的“触手”行为是通过流体系统中的各种溶剂发现的。