Mesenchymal stem cells (MSCs) have immense therapeutic potential because of their ability to self-renew and differentiate into various connective tissue lineages. The in vitro proliferation and expansion of these cells is necessary for their use in stem cell therapy. Recently our group has developed and characterized mesenchymal stem cells from subcutaneous and visceral adipose tissue. We observed that these cells show a slower growth rate at higher passages and therefore decided to develop a supplemented medium, which will induce proliferation. Choi et al. have recently shown that the use of ascorbic acid enhances the proliferation of bone marrow derived MSCs. We therefore studied the effect of ascorbic acid on the proliferation of MSCs and characterized their phenotypes using stem cell specific molecular markers. It was observed that the use of 250 μM ascorbic acid promoted the significant growth of MSCs without loss of phenotype and differentiation potential. There was no considerable change in gene expression of cell surface markers CD105, CD13, Nanog, leukemia inhibitory factor (LIF) and Keratin 18. Moreover, the MSCs maintained in the medium supplemented with ascorbic acid for a period of 4 weeks showed increase in pluripotency markers Oct4 and SOX 2. Also cells in the experimental group retained the typical spindle shaped morphology. Thus, this study emphasizes the development of suitable growth medium for expansion of MSCs and maintenance of their undifferentiated state for further therapeutic use.

中文翻译：

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抗坏血酸通过上调胚胎干细胞多能性标记物 Oct4 和 SOX 2 诱导人皮下脂肪组织来源的间充质干细胞的体外增殖。

间充质干细胞 (MSCs) 具有巨大的治疗潜力，因为它们具有自我更新和分化成各种结缔组织谱系的能力。在体外，这些细胞的增殖和扩展是必要的其在干细胞治疗中的用途。最近，我们的团队开发并表征了来自皮下和内脏脂肪组织的间充质干细胞。我们观察到这些细胞在更高的传代中显示出较慢的生长速度，因此决定开发一种补充培养基，这将诱导增殖。崔等人. 最近表明，抗坏血酸的使用增强了骨髓来源的 MSC 的增殖。因此，我们研究了抗坏血酸对 MSC 增殖的影响，并使用干细胞特异性分子标记表征了它们的表型。据观察，250 μM 抗坏血酸的使用促进了 MSC 的显着生长，而不会丧失表型和分化潜力。细胞表面标志物 CD105、CD13、Nanog、白血病抑制因子 (LIF) 和角蛋白 18 的基因表达没有显着变化。此外，在补充抗坏血酸的培养基中维持 4 周的 MSC 显示多能性增加标记物 Oct4 和 SOX 2。实验组中的细胞也保留了典型的纺锤形形态。因此，