We have studied the formation and the stability of ionomer complexes from grafted copolymers (GICs) in solution and the influence of GIC coatings on the adsorption of the proteins β-lactoglobulin (β-lac), bovine serum albumin (BSA), and lysozyme (Lsz) on silica and polysulfone. The GICs consist of the grafted copolymer PAA28-co-PAPEO22 {poly(acrylic acid)-co-poly[acrylate methoxy poly(ethylene oxide)]} with negatively charged AA and neutral APEO groups, and the positively charged homopolymers: P2MVPI43 [poly(N-methyl 2-vinyl pyridinium iodide)] and PAH∙HCl160 [poly(allylamine hydrochloride)]. In solution, these aggregates are characterized by means of dynamic and static light scattering. They appear to be assemblies with hydrodynamic radii of 8 nm (GIC-PAPEO22/P2MVPI43) and 22 nm (GIC-PAPEO22/PAH∙HCl160), respectively. The GICs partly disintegrate in solution at salt concentrations above 10 mM NaCl. Adsorption of GICs and proteins has been studied with fixed angle optical reflectometry at salt concentrations ranging from 1 to 50 mM NaCl. Adsorption of GICs results in high density PEO side chains on the surface. Higher densities were obtained for GICs consisting of PAH∙HCl160 (1.6 ÷ 1.9 chains/nm2) than of P2MVPI43 (0.6 ÷ 1.5 chains/nm2). Both GIC coatings strongly suppress adsorption of all proteins on silica (>90%); however, reduction of protein adsorption on polysulfone depends on the composition of the coating and the type of protein. We observed a moderate reduction of β-lac and Lsz adsorption (>60%). Adsorption of BSA on the GIC-PAPEO22/P2MVPI43 coating is moderately reduced, but on the GIC-PAPEO22/PAH∙HCl160 coating it is enhanced.

中文翻译：

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接枝离聚物复合物及其对二氧化硅和聚砜表面上蛋白质吸附的影响

我们研究了溶液中接枝共聚物 (GIC) 离聚物复合物的形成和稳定性，以及 GIC 涂层对蛋白质 β-乳球蛋白 (β-lac)、牛血清白蛋白 (BSA) 和溶菌酶吸附的影响。 Lsz) 在二氧化硅和聚砜上。GIC 由带负电荷的 AA 和中性 APEO 基团的接枝共聚物 PAA28-co-PAPEO22 {聚（丙烯酸）-共聚[丙烯酸酯甲氧基聚（环氧乙烷）]}和带正电荷的均聚物：P2MVPI43 [聚（N-甲基 2-乙烯基吡啶碘化物）] 和 PAH∙HCl160 [聚（烯丙胺盐酸盐）]。在溶液中，这些聚集体通过动态和静态光散射来表征。它们似乎是流体力学半径分别为 8 nm (GIC-PAPEO22/P2MVPI43) 和 22 nm (GIC-PAPEO22/PAH∙HCl160) 的组件。当盐浓度高于 10 mM NaCl 时，GIC 在溶液中部分分解。已使用固定角度光学反射计在 1 至 50 mM NaCl 的盐浓度范围内研究了 GIC 和蛋白质的吸附。GIC 的吸附导致表面上的高密度 PEO 侧链。由 PAH∙HCl160 (1.6 ÷ 1.9 链/nm2) 组成的 GIC 的密度高于 P2MVPI43 (0.6 ÷ 1.5 链/nm2)。两种 GIC 涂层都强烈抑制所有蛋白质在硅胶上的吸附 (>90%)；然而，聚砜上蛋白质吸附的减少取决于涂层的组成和蛋白质的类型。我们观察到 β-lac 和 Lsz 吸附适度减少（>60%）。BSA 在 GIC-PAPEO22/P2MVPI43 涂层上的吸附适度减少，但在 GIC-PAPEO22/PAH∙HCl160 涂层上的吸附增强。