We outline the near 50-year history of leukemia-lymphoma (LL) cell lines — a key model system in biomedicine. Due to the detailed documentation of their oncogenomic and transcriptional alterations via recent advances in molecular medicine, LL cell lines may be fitted to parent tumors with a degree of precision unattainable in other cancers. We have surveyed the corpus of published LL cell lines and found 637 examples that meet minimum standards of authentication and characterization. Alarmingly, the rate of establishment of new LL cell lines has plummeted over the last decade. Although the main hematopoietic developmental cell types are represented by cell lines, some LL categories stubbornly resist establishment in vitro. The advent of engineering techniques for immortalizing primary human cells that maintain differentiation means the time is ripe for renewed search for in vitro models from un(der)represented hematologic entities. Given their manifold applications in biomedicine, there is little doubt that LL-derived cell lines will continue to play a vital part well into the next half-century as well.

中文翻译：

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白血病淋巴瘤细胞系的历史。

我们概述了白血病-淋巴瘤 (LL) 细胞系近 50 年的历史——生物医学中的一个关键模型系统。由于分子医学的最新进展详细记录了它们的致癌基因组和转录改变，因此 LL 细胞系可能会以其他癌症无法达到的精确度适应亲本肿瘤。我们调查了已发表的 LL 细胞系的语料库，发现了 637 个符合最低认证和表征标准的例子。令人震惊的是，在过去十年中，新 LL 细胞系的建立速度急剧下降。虽然主要的造血发育细胞类型以细胞系为代表，但一些 LL 类别顽固地抵抗体外建立. 用于使维持分化的原代人类细胞永生化的工程技术的出现意味着重新寻找来自未代表的血液学实体的体外模型的时机已经成熟。鉴于它们在生物医学中的多种应用，毫无疑问，LL 衍生的细胞系也将在接下来的半个世纪中继续发挥重要作用。