Improvement of soft tissue sarcoma patient outcome requires well-characterized animal models in which to evaluate novel therapeutic options. Xenograft sarcoma models are frequently used, but commonly with established cell lines rather than with primary human sarcoma cells. The objective of the present study was to establish a reproducible xenograft model of primary human soft tissue sarcoma in athymic nude mice. Primary soft tissue sarcoma cells from four resected human sarcomas were isolated, cultured until the third passage and injected subcutaneously into athymic nude mice. The sarcoma xenograft was further analyzed by histological and immunohistochemical staining. In two out of four sarcomas tumor growth could successfully be established leading to solid tumors of up to 540 mm³ volume. Histological and immunohistochemical staining confirmed the mouse xenograft as identical sarcoma compared with the original patient’s tissue. In the present study a reproducible xenograft model of primary human soft tissue sarcoma in athymic nude mice was established. This animal model is of great interest for the study of sarcomogenesis and therapy.

中文翻译：

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无胸腺裸鼠原代人肉瘤模型的建立。

软组织肉瘤患者预后的改善需要特征明确的动物模型来评估新的治疗方案。异种移植肉瘤模型经常使用，但通常用于已建立的细胞系，而不是原代人类肉瘤细胞。本研究的目的是在无胸腺裸鼠中建立可重复的原发性人类软组织肉瘤异种移植模型。从四个切除的人肉瘤中分离原代软组织肉瘤细胞，培养至第三代并皮下注射到无胸腺裸鼠中。通过组织学和免疫组织化学染色进一步分析肉瘤异种移植物。在四分之二的肉瘤中，可以成功地建立肿瘤生长，导致高达 540 mm ^{3 的}实体瘤体积。组织学和免疫组织化学染色证实，与原始患者的组织相比，小鼠异种移植物是相同的肉瘤。在本研究中，在无胸腺裸鼠中建立了可重复的原发性人类软组织肉瘤异种移植模型。这种动物模型对肉瘤发生和治疗的研究具有重要意义。