We have studied the formation and the stability of grafted block complex coacervate core micelles (C3Ms) in solution and the influence of grafted block C3M coatings on the adsorption of the proteins β-lactoglobulin, bovine serum albumin, and lysozyme. The C3Ms consist of a grafted block copolymer PAA21-b-PAPEO14 (poly(acrylic acid)-b-poly(acrylate methoxy poly(ethylene oxide)), with a negatively charged PAA block and a neutral PAPEO block and a positively charged homopolymer P2MVPI (poly(N-methyl 2-vinyl pyridinium iodide). In solution, these C3Ms partly disintegrate at salt concentrations between 50 and 100 mM NaCl. Adsorption of C3Ms and proteins has been studied with fixed-angle optical reflectometry, at salt concentrations ranging from 1 to 100 mM NaCl. In comparison with the adsorption of PAA21-b-PAPEO14 alone adsorption of C3Ms significantly increases the amount of PAA21-b-PAPEO14 on the surface. This results in a higher surface density of PEO chains. The stability of the C3M coatings and their influence on protein adsorption are determined by the composition and the stability of the C3Ms in solution. A C3M-PAPEO14/P2MVPI43 coating strongly suppresses the adsorption of all proteins on silica and polystyrene. The reduction of protein adsorption is the highest at 100 mM NaCl (>90%). The adsorbed C3M-PAPEO14/P2MVPI43 layer is partly removed from the surface upon exposure to an excess of β-lactoglobulin solution, due to formation of soluble aggregates consisting of β-lactoglobulin and P2MVPI43. In contrast, C3M-PAPEO14/P2MVPI228 which has a fivefold longer cationic block enhances adsorption of the negatively charged proteins on both surfaces at salt concentrations above 1 mM NaCl. A single PAA21-b-PAPEO14 layer causes only a moderate reduction of protein adsorption.

中文翻译：

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接枝块复合凝聚核胶束及其对二氧化硅和聚苯乙烯上蛋白质吸附的影响

我们研究了溶液中接枝块复合凝聚核心胶束 (C3Ms) 的形成和稳定性，以及接枝块 C3M 涂层对蛋白质 β-乳球蛋白、牛血清白蛋白和溶菌酶吸附的影响。C3M 由接枝嵌段共聚物 PAA21-b-PAPEO14（聚（丙烯酸）-b-聚（丙烯酸酯甲氧基聚（环氧乙烷））、带负电荷的 PAA 嵌段和中性 PAPEO 嵌段以及带正电荷的均聚物 P2MVPI 组成（聚（N-甲基 2-乙烯基吡啶碘化物）。在溶液中，这些 C3Ms 在 50 到 100 mM NaCl 的盐浓度下部分分解。已使用固定角光学反射计研究了 C3Ms 和蛋白质的吸附，盐浓度范围为1 至 100 mM 氯化钠。与单独吸附 PAA21-b-PAPEO14 相比，C3Ms 的吸附显着增加了表面上 PAA21-b-PAPEO14 的数量。这导致 PEO 链的表面密度更高。C3M 涂层的稳定性及其对蛋白质吸附的影响取决于 C3M 在溶液中的组成和稳定性。C3M-PAPEO14/P2MVPI43 涂层强烈抑制所有蛋白质在二氧化硅和聚苯乙烯上的吸附。蛋白质吸附的减少在 100 mM NaCl (>90%) 时最高。由于形成由 β-乳球蛋白和 P2MVPI43 组成的可溶性聚集体，吸附的 C3M-PAPEO14/P2MVPI43 层在暴露于过量的 β-乳球蛋白溶液后会部分从表面去除。相比之下，C3M-PAPEO14/P2MVPI228 具有五倍长的阳离子嵌段，可在盐浓度高于 1 mM NaCl 时增强带负电荷的蛋白质在两个表面上的吸附。单个 PAA21-b-PAPEO14 层仅会适度减少蛋白质吸附。