The trans-lycopene content of fresh tomato homogenates was assessed by means of the laser photoacoustic spectroscopy, the laser optothermal window, micro-Raman spectroscopy, and colorimetry; none of these methods require the extraction from the product matrix prior to the analysis. The wet chemistry method (high-performance liquid chromatography) was used as the absolute quantitative method. Analytical figures of merit for all methods were compared statistically; best linear correlation was achieved for the chromaticity index a* and chroma C*.

中文翻译：

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通过 HPLC 方法测量反式番茄红素与光热和光声信号以及新鲜番茄匀浆的颜色读数的相关性。

新鲜番茄匀浆的反式番茄红素含量通过激光光声光谱、激光光热窗、显微拉曼光谱和比色法进行评估；这些方法都不需要在分析前从产品矩阵中提取。湿化学法（高效液相色谱法）用作绝对定量方法。对所有方法的分析优值进行了统计比较；色度指数a * 和色度C *实现了最佳线性相关性。