Diamagnetic samples placed in a strong magnetic field and a magnetic field gradient experience a magnetic force. Stable magnetic levitation occurs when the magnetic force exactly counter balances the gravitational force. Under this condition, a diamagnetic sample is in a simulated microgravity environment. The purpose of this study is to explore if MC3T3-E1 osteoblastic cells can be grown in magnetically simulated hypo-g and hyper-g environments and determine if gene expression is differentially expressed under these conditions. The murine calvarial osteoblastic cell line, MC3T3-E1, grown on Cytodex-3 beads, were subjected to a net gravitational force of 0, 1 and 2 g in a 17 T superconducting magnet for 2 days. Microarray analysis of these cells indicated that gravitational stress leads to up and down regulation of hundreds of genes. The methodology of sustaining long-term magnetic levitation of biological systems are discussed.

中文翻译：

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MC3T3 成骨细胞的磁悬浮作为微重力的地面模拟。

置于强磁场和磁场梯度中的抗磁性样品会受到磁力的影响。当磁力正好抵消重力时，就会发生稳定的磁悬浮。在这种情况下，抗磁性样品处于模拟的微重力环境中。本研究的目的是探索 MC3T3-E1 成骨细胞是否可以在磁性模拟的低重力和超重力环境中生长，并确定基因表达在这些条件下是否有差异表达。在 Cytodex-3 珠上生长的鼠颅骨成骨细胞系 MC3T3-E1 在 17 T 超导磁体中承受 0、1 和 2 g 的净重力，持续 2 天。这些细胞的微阵列分析表明，重力应力导致数百个基因的上调和下调。