The tree-legume Leucaena leucocephala (leucaena) is used as a perennial fodder because of its fast-growing foliage, which is high in protein content. The use of leucaena as a fodder is however restricted due to the presence of the toxin mimosine. Improvements in the nutritional contents as well as other agronomic traits of leucaena can be accomplished through genetic transformation. The objective of this research was to develop a transformation protocol for leucaena using phosphinothricin resistance as the plant selectable marker. Explants obtained from immature zygotic embryos infected with the Agrobacterium tumefaciens strain C58C1 containing the binary plasmid pCAMBIA3201 produced four putative transformed leucaena plants. Transformation was con- firmed by PCR, RT-PCR, Southern blot, Western analyses, GUS-specific enzyme activity and herbicide leaf spraying assay. A transformation efficiency of 2% was established using this protocol.

中文翻译：

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使用未成熟的合子胚胎为树豆科 Leucaena leucocephala 开发农杆菌介导的转化方案。

豆科植物银合欢（leucaena）因其生长速度快、蛋白质含量高而被用作多年生饲料。然而，由于含羞草毒素的存在，银合欢作为饲料的使用受到限制。银合欢的营养成分和其他农艺性状的改进可以通过遗传转化来实现。本研究的目的是开发一种使用草胺膦抗性作为植物选择标记的银合欢转化方案。从用含有二元质粒 pCAMBIA3201 的根癌农杆菌 C58C1 菌株感染的未成熟合子胚获得的外植体产生了四种假定的转化银合欢植物。转化通过 PCR、RT-PCR、Southern 印迹、Western 分析、GUS 特异性酶活性和除草剂叶片喷洒试验。使用该协议建立了 2% 的转化效率。