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CRISPR elements in the Thermococcales: evidence for associated horizontal gene transfer in Pyrococcus furiosus.
Journal of Applied Genetics ( IF 2.4 ) Pub Date : 2009 , DOI: 10.1007/bf03195703
M C Portillo 1 , J M Gonzalez
Affiliation  

The presence and distribution of CRISPR (clustered regularly interspaced short palindrome repeat) elements in the archaeal order Thermococcales were analyzed. Four complete genome sequences from the speciesPyrococcus abyssi, P. furiosus, P. horikoshii, andThermococcus kodakaraensis were studied. A fragment of the genome ofP. furiosus was flanked by CRISPR elements upstream and by a single element downstream. The composition of the gene sequences contained in this genome fragment (positions 699013 to 855319) showed significant differences from the other genes in theP. furiosus genome. Differences were observed in the GC content at the third codon positions and the frequency of codon usage between the genes located in the analyzed fragment and the other genes in theP. furiosus genome. These results represent the first evidence suggesting that repeated CRISPR elements can be involved in horizontal gene transfer and genomic differentiation of hyperthermophilic Archaea.

中文翻译:

Thermococcales 中的 CRISPR 元件:Pyrococcus furiosus 中相关水平基因转移的证据。

分析了古菌目 Thermococcales 中 CRISPR(成簇的规则间隔短回文重复序列)元素的存在和分布。研究了来自Pyrococcus abyssiP. furiosus、P. horikoshiiThermococcus kodakaraensis 的四种完整基因组序列。P. furiosus基因组的一个片段的两侧是 CRISPR 元件上游和单个元件下游。该基因组片段中包含的基因序列的组成(位置 699013 至 855319)与P. furiosus 中的其他基因有显着差异基因组。在第三个密码子位置的 GC 含量以及位于分析片段中的基因与P. furiosus基因组中的其他基因之间的密码子使用频率方面观察到差异。这些结果代表了第一个证据,表明重复的 CRISPR 元件可以参与超嗜热古细菌的水平基因转移和基因组分化。
更新日期:2020-09-22
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