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Correlation between porcine PPARGC1A mRNA expression and its downstream target genes in backfat and longissimus dorsi muscle.
Journal of Applied Genetics ( IF 2.4 ) Pub Date : 2009 , DOI: 10.1007/bf03195694
T Erkens 1 , J Vandesompele , A Van Zeveren , L J Peelman
Affiliation  

Knowledge of in vivo relationship between the coactivatorPPARGC1A and its target genes is very limited, especially in the pig. In this study, a real-time PCR experiment was performed onlongissimus dorsi muscle (MLD) and backfat with 10 presumedPPARGC1A downstream target genes, involved in energy and fat metabolism, to identify possible relationships withPPARGC1A mRNA expression in vivo in the pig (n = 20). Except forUCP3 andLPL, a very significant difference in expression was found between MLD and backfat for all genes (P < 0.01). Hierarchical cluster analysis and the significant pairing of mRNA expression data between sampling locations suggested a genetic regulation of the expression of several target genes. A positive correlation withPPARGC1A was found forCPT1B, GLUT4, PDK4, andTFAM (P < 0.0001). A negative correlation was found forUCP2, FABP4, LEP (P < 0.0001), andTNF (P = 0.0071). No significant correlation was detected forUCP3 andLPL. This study provides evidence for a clear difference in mRNA expression of crucial genes in fat and energy metabolism between 2 important tissues. Our data suggest a clear impact ofPPARGC1A on energy and lipid metabolism in vivo in the pig, through several of these downstream target genes.

中文翻译:

猪背膘和背最长肌中PPARGC1A mRNA表达与其下游靶基因的相关性。

对共激活因子PPARGC1A与其靶基因之间的体内关系的了解非常有限,尤其是在猪中。在本研究中,对背最长肌(MLD) 和背膘进行了实时 PCR 实验,其中包含 10 个推测的PPARGC1A下游靶基因,涉及能量和脂肪代谢,以确定与猪体内PPARGC1A mRNA 表达的可能关系。n = 20)。除UCP3LPL 外,MLD 和背膘的所有基因的表达差异都非常显着(P< 0.01)。分层聚类分析和采样位置之间 mRNA 表达数据的显着配对表明几种靶基因表达的遗传调节。发现CPT1B、GLUT4、PDK4TFAMPPARGC1A呈正相关(P < 0.0001)。发现UCP2、FABP4、LEP ( P < 0.0001) 和TNF (P = 0.0071)呈负相关。未检测到UCP3LPL 的显着相关性。该研究为两种重要组织之间脂肪和能量代谢中关键基因的 mRNA 表达存在明显差异提供了证据。我们的数据表明PPARGC1A对猪体内能量和脂质代谢的影响,通过这些下游靶基因中的几个。
更新日期:2020-09-22
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