The integration of near IR picosecond pulse excitation, collinear beam geometry, epi-detection, and laser-scanning has produced a coherent anti-Stokes Raman scattering (CARS) microscope with a detection sensitivity of 10(5) vibrational oscillators, sub-micron 3D resolution, and video-rate acquisition speed. The incorporation of spectral detection and other imaging modalities has added versatility to the CARS microscope. These advances allowed sensitive interrogation of biological samples, particularly lipids that have a high density of CH(2) groups. With initial applications to membrane domains, lipid bodies, demyelinating diseases, obesity, and cardiovascular diseases, CARS microscopy is poised to become a powerful bio-imaging tool with the availability of a multifunctional, affordable, easy-to-operate CARS microscope, and the development of CARS endoscopy for in vivo diagnosis.

中文翻译：

---

通过相干反斯托克斯拉曼散射显微镜追踪健康和疾病中的脂质

近红外皮秒脉冲激发、共线光束几何结构、落射检测和激光扫描的集成产生了相干反斯托克斯拉曼散射 (CARS) 显微镜，其检测灵敏度为 10(5) 个振动振荡器、亚微米 3D分辨率和视频速率采集速度。光谱检测和其他成像方式的结合增加了 CARS 显微镜的多功能性。这些进步允许对生物样品进行灵敏的询问，特别是具有高密度 CH(2) 基团的脂质。随着最初应用于膜域、脂质体、脱髓鞘疾病、肥胖和心血管疾病，CARS 显微镜有望成为一种强大的生物成像工具，具有多功能、价格实惠、易于操作的 CARS 显微镜和用于体内诊断的 CARS 内窥镜的发展。